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dc.contributor.author
Correia, Cláudia
dc.contributor.author
Koshkin, Alexey
dc.contributor.author
Duarte, Patrícia
dc.contributor.author
Hu, Dongjian
dc.contributor.author
Teixeira, Ana
dc.contributor.author
Domian, Ibrahim
dc.contributor.author
Serra, Margarida
dc.contributor.author
Alves, Paula M.
dc.date.accessioned
2017-11-22T15:49:52Z
dc.date.available
2017-10-06T03:23:52Z
dc.date.available
2017-11-22T15:49:52Z
dc.date.issued
2017-08-17
dc.identifier.issn
2045-2322
dc.identifier.other
10.1038/s41598-017-08713-4
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/191559
dc.identifier.doi
10.3929/ethz-b-000191559
dc.description.abstract
The immature phenotype of human pluripotent stem cell derived cardiomyocytes (hPSC-CMs) constrains their potential in cell therapy and drug testing. In this study, we report that shifting hPSC-CMs from glucose-containing to galactose- and fatty acid-containing medium promotes their fast maturation into adult-like CMs with higher oxidative metabolism, transcriptional signatures closer to those of adult ventricular tissue, higher myofibril density and alignment, improved calcium handling, enhanced contractility, and more physiological action potential kinetics. Integrated “-Omics” analyses showed that addition of galactose to culture medium improves total oxidative capacity of the cells and ameliorates fatty acid oxidation avoiding the lipotoxicity that results from cell exposure to high fatty acid levels. This study provides an important link between substrate utilization and functional maturation of hPSC-CMs facilitating the application of this promising cell type in clinical and preclinical applications.
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Cardiac regeneration
en_US
dc.subject
Data integration
en_US
dc.subject
Induced pluripotent stem cells
en_US
dc.subject
Stem-cell research
en_US
dc.title
Distinct carbon sources affect structural and functional maturation of cardiomyocytes derived from human pluripotent stem cells
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
Scientific Reports
ethz.journal.volume
7
en_US
ethz.pages.start
8590
en_US
ethz.size
17 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.status
published
en_US
ethz.date.deposited
2017-10-06T03:24:15Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-11-22T15:50:01Z
ethz.rosetta.lastUpdated
2020-02-15T09:38:49Z
ethz.rosetta.versionExported
true
ethz.COinS
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