Arzhanik, Vladimir K.
Drobyshev, Alexei L.
Ataullakhanov, Fazly I.
Volkov, Vladimir A.
- Journal Article
Rights / licenseCreative Commons Attribution-NonCommercial 3.0 Unported
Dynamic microtubule ends exert pulling and pushing forces on intracellular membranes and organelles. However, the mechanical linkage of microtubule tips to their cargoes is poorly understood. CENP-F is a nonmotor microtubule-binding protein that participates in microtubule binding at kinetochores and in the mitotic redistribution of the mitochondrial network. CENP-F–driven mitochondrial transport is linked to growing microtubule tips, but the underlying molecular mechanisms are unknown. Here we show that CENP-F tracks growing microtubule ends in living cells. In vitro reconstitution demonstrates that microtubule tips can transport mitochondria and CENP-F–coated artificial cargoes over micrometer-long distances during both growing and shrinking phases. Based on these and previous observations, we suggest that CENP-F might act as a transporter of mitochondria and other cellular cargoes by attaching them to dynamic microtubule ends during both polymerization and depolymerization of tubulin. Show more
Journal / seriesMolecular Biology of the Cell
Pages / Article No.
PublisherAmerican Society for Cell Biology
Organisational unit03923 - Kornmann, Benoît (SNF-Professur) (ehem.) / Kornmann, Benoît (SNF-Professur) (form.)
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