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dc.contributor.author
Garcia-Alles, Luis F.
dc.contributor.author
Lesniewska, Eric
dc.contributor.author
Root, Katharina
dc.contributor.author
Aubry, Nathalie
dc.contributor.author
Pocholle, Nicolas
dc.contributor.author
Mendoza, Carlos I.
dc.contributor.author
Bourillot, Eric
dc.contributor.author
Barylyuk, Konstantin
dc.contributor.author
Pompon, Denis
dc.contributor.author
Zenobi, Renato
dc.contributor.author
Reguera, David
dc.contributor.author
Truan, Gilles
dc.date.accessioned
2017-12-20T17:49:51Z
dc.date.available
2017-10-06T05:17:16Z
dc.date.available
2017-12-20T17:49:51Z
dc.date.issued
2017
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0185109
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/192570
dc.identifier.doi
10.3929/ethz-b-000192570
dc.description.abstract
CcmK proteins are major constituents of icosahedral shells of β-carboxysomes, a bacterial microcompartment that plays a key role for CO2 fixation in nature. Supported by the characterization of bidimensional (2D) layers of packed CcmK hexamers in crystal and electron microscopy structures, CcmK are assumed to be the major components of icosahedral flat facets. Here, we reassessed the validity of this model by studying CcmK isoforms from Synechocystis sp. PCC6803. Native mass spectrometry studies confirmed that CcmK are hexamers in solution. Interestingly, potential pre-assembled intermediates were also detected with CcmK2. Atomic-force microscopy (AFM) imaging under quasi-physiological conditions confirmed the formation of canonical flat sheets with CcmK4. Conversely, CcmK2 formed both canonical and striped-patterned patches, while CcmK1 assembled into remarkable supra-hexameric curved honeycomb-like mosaics. Mutational studies ascribed the propensity of CcmK1 to form round assemblies to a combination of two features shared by at least one CcmK isoform in most β-cyanobacteria: a displacement of an α helical portion towards the hexamer edge, where a potential phosphate binding funnel forms between packed hexamers, and the presence of a short C-terminal extension in CcmK1. All-atom molecular dynamics supported a contribution of phosphate molecules sandwiched between hexamers to bend CcmK1 assemblies. Formation of supra-hexameric curved structures could be reproduced in coarse-grained simulations, provided that adhesion forces to the support were weak. Apart from uncovering unprecedented CcmK self-assembly features, our data suggest the possibility that transitions between curved and flat assemblies, following cargo maturation, could be important for the biogenesis of β-carboxysomes, possibly also of other BMC.
en_US
dc.format
application/pdf
dc.language.iso
en
en_US
dc.publisher
PLOS
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Spontaneous non-canonical assembly of CcmK hexameric components from β-carboxysome shells of cyanobacteria
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2017-09-21
ethz.journal.title
PLoS ONE
ethz.journal.volume
12
en_US
ethz.journal.issue
9
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e0185109
en_US
ethz.size
30 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Soft ionization mass spectrometry for studying noncovalent interactions
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
San Francisco, CA
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02514 - Laboratorium für Organische Chemie / Laboratory of Organic Chemistry::03430 - Zenobi, Renato / Zenobi, Renato
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02514 - Laboratorium für Organische Chemie / Laboratory of Organic Chemistry::03430 - Zenobi, Renato / Zenobi, Renato
ethz.grant.agreementno
159929
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Projekte MINT
ethz.date.deposited
2017-10-06T05:17:29Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-12-20T17:50:03Z
ethz.rosetta.lastUpdated
2024-02-02T03:35:01Z
ethz.rosetta.versionExported
true
ethz.COinS
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