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dc.contributor.author
Sartori, Carlotta
dc.contributor.author
Boss, R.
dc.contributor.author
Ivanovic, I.
dc.contributor.author
Graber, H.U.
dc.date.accessioned
2017-11-23T10:52:41Z
dc.date.available
2017-10-10T02:02:03Z
dc.date.available
2017-11-23T10:52:41Z
dc.date.issued
2017-10
dc.identifier.issn
0022-0302
dc.identifier.issn
1525-3198
dc.identifier.other
10.3168/jds.2017-12820
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/193532
dc.identifier.doi
10.3929/ethz-b-000193532
dc.description.abstract
The specific and reliable diagnosis of mastitis pathogens is essential for successful sanitation programs. The aim of the present study was to develop and evaluate a new real-time quantitative PCR (qPCR) assay for the very sensitive and specific detection of Staphylococcus aureus genotype B in cow milk samples. This mastitis pathogen is contagious and particularly prevalent in Switzerland and other central European countries. The new test is based on a rapid preparation of bacteria, followed by DNA isolation and qPCR for a unique target gene coding for the adhesion-like bovine protein (adlb). The inclusivity of the new target gene was 97% and the exclusivity 98%, meaning that other genotypes and bacterial species could be excluded with high reliability. The limit of detection of the new assay was 235 staphylococcal cell equivalents/mL of culture. The new test shows high intra- and interassay repeatability. Results are available within 2 d after sampling, allowing farmers and veterinarians to apply sanitation measures immediately. Based on the results of a preliminary field study, the diagnostic sensitivity and specificity of the new qPCR assay are 99 and 100%, respectively. The new analytical procedure is straightforward and can be applied for routine diagnostics.
en_US
dc.language.iso
en
en_US
dc.publisher
Elsevier
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-nd/3.0/
dc.subject
diagnostics
en_US
dc.subject
Staphylococcus aureus
en_US
dc.subject
milk
en_US
dc.subject
mastitis
en_US
dc.subject
real-time quantitative PCR
en_US
dc.title
Development of a new real-time quantitative PCR assay for the detection of Staphylococcus aureus genotype B in cow milk, targeting the new gene adlb
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported
dc.date.published
2017-07-26
ethz.journal.title
Journal of Dairy Science
ethz.journal.volume
100
en_US
ethz.journal.issue
10
en_US
ethz.journal.abbreviated
J. dairy sci.
ethz.pages.start
7834
en_US
ethz.pages.end
7845
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Savoy, IL
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2017-10-10T02:02:18Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-11-23T10:52:45Z
ethz.rosetta.lastUpdated
2018-08-03T02:57:46Z
ethz.rosetta.versionExported
true
ethz.COinS
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