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dc.contributor.author
McCormick, Andrea C.
dc.contributor.author
Grosse-Wilde, Ewald
dc.contributor.author
Wheeler, David
dc.contributor.author
Mescher, Mark C.
dc.contributor.author
Hansson, Bill S.
dc.contributor.author
De Moraes, Consuelo M.
dc.date.accessioned
2017-12-18T14:05:48Z
dc.date.available
2017-10-28T02:35:36Z
dc.date.available
2017-12-18T14:05:48Z
dc.date.issued
2017-09
dc.identifier.other
10.3389/fevo.2017.00115
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/201936
dc.identifier.doi
10.3929/ethz-b-000201936
dc.description.abstract
In insects, flight and sophisticated olfactory systems go hand in hand and are essential to survival and evolutionary success. Females of many Lepidopteran species have secondarily lost their flight ability, which may lead to changes in the olfactory capabilities of both larval and adult stages. The gypsy moth, Lymantria dispar, an important forest pest worldwide, is currently undergoing a diversification process with three recognized subspecies: the Asian gypsy moth (AGM), Lymantria dispar asiatica; the Japanese gypsy moth (JGM), Lymantria dispar japonica; and the European gypsy moth (EGM), Lymantria dispar dispar. Females of EGM populations from North America have lost their flight capacity whereas the JGM and AGM females are flight capable, making this an ideal system to investigate the relationship between flight and olfaction. We used next-generation sequencing to obtain female antennal and larval head capsule transcriptomes in order to (i) investigate the differences in expression of olfaction-related genes among populations; (ii) identify the most similar protein sequences reported for other organisms through a BLAST search, and (iii) establish the phylogenetic relationships of these sequences with respect to other insect species. Using this approach, we identified 115 putative chemosensory genes belonging to five families of olfaction-related genes. A principal component analysis (PCA) revealed that the gene-expression patterns of female antennal transcriptomes from different subspecies were more similar to one another than to the larval head capsules of their respective subspecies supporting strong chemosensory differences between the two developmental stages. An analysis of the shared and exclusively expressed genes for three populations shows no evidence that loss of flight affects the number or type of genes being expressed. These results indicate either (a) that loss of flight does not impact the olfactory gene repertoire or (b) that the secondary loss of flight in American EGM populations may be too recent to have caused major changes in the genes being expressed. However, we found higher expression values for most olfaction-related genes in EGM females, suggesting that differences in transcription rates could be an adaptation of flightless females to their chemical environment. Differences in olfactory genes and their expression in the larvae appear to be unrelated to the flight ability of adult females and are likely adaptations to different ecological pressures.
en_US
dc.language.iso
en
en_US
dc.publisher
Frontiers Media
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Lymantria dispar
en_US
dc.subject
transcriptome
en_US
dc.subject
odorant receptor
en_US
dc.subject
ionotropic receptor
en_US
dc.subject
gustatory receptor
en_US
dc.subject
odorant binding protein
en_US
dc.subject
chemosensory protein
en_US
dc.title
Comparing the expression of olfaction-related genes in gypsy moth (Lymantria dispar) adult females and larvae from one flightless and two flight-capable populations
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2017-09-29
ethz.journal.title
Frontiers in Ecology and Evolution
ethz.journal.volume
5
en_US
ethz.pages.start
115
en_US
ethz.size
19 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.scopus
ethz.publication.place
Lausanne
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::03909 - Levine, Jonathan M. / Levine, Jonathan M.
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03970 - De Moraes, Consuelo / De Moraes, Consuelo
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::03909 - Levine, Jonathan M. / Levine, Jonathan M.
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03970 - De Moraes, Consuelo / De Moraes, Consuelo
en_US
ethz.date.deposited
2017-10-28T02:35:38Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-12-18T14:05:55Z
ethz.rosetta.lastUpdated
2018-02-01T10:10:05Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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