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dc.contributor.author
Krawczynski, Kamil
dc.contributor.author
Bauersachs, Stefan
dc.contributor.author
Reliszko, Zaneta P.
dc.contributor.author
Graf, Alexander
dc.contributor.author
Kaczm, Monika M.
dc.date.accessioned
2021-03-01T13:25:09Z
dc.date.available
2017-06-14T03:39:18Z
dc.date.available
2017-07-11T11:34:32Z
dc.date.available
2017-06-11T20:52:55Z
dc.date.available
2017-11-13T12:43:40Z
dc.date.available
2021-03-01T13:25:09Z
dc.date.issued
2015-11-06
dc.identifier.other
10.1186/s12864-015-2172-2
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/207385
dc.identifier.doi
10.3929/ethz-a-010580453
dc.description.abstract
Background Embryo implantation is a complex, synchronized process that requires establishment of a reciprocal dialogue between a receptive endometrium and developing blastocysts. Recently, microRNAs (miRNAs), known to modulate gene expression through post-transcriptional mechanisms, were implicated in regulation of early pregnancy events including maternal recognition of pregnancy and implantation. To characterize complex transcriptomic changes, expression of miRNAs in pregnant and cyclic endometria collected on days 12, 16 and 20 was analyzed using Illumina deep sequencing and analyzed with bioinformatic pipeline. Moreover, expression profiles of ten genes related to miRNA synthesis and transport such as DROSHA, DGCR8, XPO5, DICER, TARBP2, TNRC6A, and AGO1-4 were determined. Results Among genes involved in miRNA transport and synthesis DROSHA, XPO5, DICER1, TARBP, and AGO1 expression was affected by the reproductive status. Moreover, DICER1 and AGO2 proteins were localized in luminal and glandular epithelium with immunofluorescence staining. Several hundred mature, canonical and non-canonical miRNAs were found to be expressed in the endometrial samples. Detailed analysis revealed that miRNA length variants, isomiRs, accounted for the vast majority of defined sequences. Both miRNA and isomiR of miR-140-3p were shown to affect expression of putative targets in endometrial stromal cells in vitro. Computational analysis of putative target genes for miRNAs differentially expressed (DE) between pregnant and cyclic animals resulted in lists of biological processes and regulatory pathways indicating their role in cellular development, cell cycle, immunological response and organismal development. Among predicted target genes for DE miRNAs, vascular endothelial growth factor (VEGF), progesterone and estradiol receptors (PGR, ESR1) and leukemia inhibitory factor (LIF) were found. Conclusions This research revealed a repertoire of pregnancy-related miRNAs in porcine endometrium during initial stages of conceptus implantation and during the estrous cycle, and sheds light on mechanisms regulating miRNA-mediated gene expression at the maternal-conceptus interface.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
BioMed Central
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
miRNA
en_US
dc.subject
IsomiR
en_US
dc.subject
Deep sequencing
en_US
dc.subject
Endometrium
en_US
dc.subject
Implantation
en_US
dc.subject
Pig
en_US
dc.title
Expression of microRNAs and isomiRs in the porcine endometrium: implications for gene regulation at the maternal-conceptus interface
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2015-11-06
ethz.journal.title
BMC Genomics
ethz.journal.volume
16
en_US
ethz.pages.start
906
en_US
ethz.size
19 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.code.ddc
DDC - DDC::6 - Technology, medicine and applied sciences::610 - Medical sciences, medicine
en_US
ethz.code.ddc
DDC - DDC::5 - Science::590 - Zoological sciences
en_US
ethz.identifier.scopus
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03999 - Ulbrich, Susanne / Ulbrich, Susanne
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03999 - Ulbrich, Susanne / Ulbrich, Susanne
ethz.date.deposited
2017-06-11T20:53:15Z
ethz.source
ECOL
ethz.source
ECIT
ethz.identifier.importid
imp59366b853429179985
ethz.identifier.importid
imp593653aa9895972536
ethz.ecolpid
eth:48520
ethz.ecitpid
pub:166866
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-11-13T12:39:16Z
ethz.rosetta.lastUpdated
2021-02-14T20:10:21Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/155398
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/106607
ethz.COinS
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