Show simple item record

dc.contributor.author
Liv, Nalan
dc.contributor.author
Zonnevylle, A. Christiaan
dc.contributor.author
Narvaez, Angela C.
dc.contributor.author
Effting, Andries P. J.
dc.contributor.author
Voorneveld, Philip W.
dc.contributor.author
Lucas, Miriam S.
dc.contributor.author
Hardwick, James C.
dc.contributor.author
Wepf, Roger A.
dc.contributor.author
Kruit, Pieter
dc.contributor.author
Hoogenboom, Jacob P.
dc.date.accessioned
2017-11-22T06:57:42Z
dc.date.available
2017-06-10T14:51:11Z
dc.date.available
2017-11-21T14:12:54Z
dc.date.available
2017-10-31T10:15:26Z
dc.date.available
2017-11-21T14:00:00Z
dc.date.available
2017-11-22T06:57:42Z
dc.date.issued
2013-02-08
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0055707
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/211798
dc.identifier.doi
10.3929/ethz-b-000064633
dc.description.abstract
Correlative light and electron microscopy (CLEM) is a unique method for investigating biological structure-function relations. With CLEM protein distributions visualized in fluorescence can be mapped onto the cellular ultrastructure measured with electron microscopy. Widespread application of correlative microscopy is hampered by elaborate experimental procedures related foremost to retrieving regions of interest in both modalities and/or compromises in integrated approaches. We present a novel approach to correlative microscopy, in which a high numerical aperture epi-fluorescence microscope and a scanning electron microscope illuminate the same area of a sample at the same time. This removes the need for retrieval of regions of interest leading to a drastic reduction of inspection times and the possibility for quantitative investigations of large areas and datasets with correlative microscopy. We demonstrate Simultaneous CLEM (SCLEM) analyzing cell-cell connections and membrane protrusions in whole uncoated colon adenocarcinoma cell line cells stained for actin and cortactin with AlexaFluor488. SCLEM imaging of coverglass-mounted tissue sections with both electron-dense and fluorescence staining is also shown.
en_US
dc.format
application/pdf
dc.language.iso
en
en_US
dc.publisher
Public Library Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Simultaneous Correlative Scanning Electron and High-NA Fluorescence Microscopy
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2013-02-08
ethz.journal.title
PLoS ONE
ethz.journal.volume
8
en_US
ethz.journal.issue
2
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e55707
en_US
ethz.size
9 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.publication.place
San Francisco, CA
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung / Domain VP Research::02891 - ScopeM / ScopeM
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung / Domain VP Research::02891 - ScopeM / ScopeM
en_US
ethz.date.deposited
2017-06-10T14:53:16Z
ethz.source
ECIT
ethz.source
BATCH
ethz.identifier.importid
imp5936506cd7ca644673
ethz.ecitpid
pub:102747
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-11-22T06:53:46Z
ethz.rosetta.lastUpdated
2021-02-14T20:37:42Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/202732
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/64633
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Simultaneous%20Correlative%20Scanning%20Electron%20and%20High-NA%20Fluorescence%20Microscopy&rft.jtitle=PLoS%20ONE&rft.date=2013-02-08&rft.volume=8&rft.issue=2&rft.spage=e55707&rft.issn=1932-6203&rft.au=Liv,%20Nalan&Zonnevylle,%20A.%20Christiaan&Narvaez,%20Angela%20C.&Effting,%20Andries%20P.%20J.&Voorneveld,%20Philip%20W.&rft.genre=article&rft_id=info:doi/10.1371/journal.pone.0055707&
 Search print copy at ETH Library

Files in this item

Thumbnail

Publication type

Show simple item record