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dc.contributor.author
Ortner, Franziska
dc.contributor.author
Wiemeyer, Helena
dc.contributor.author
Mazzotti, Marco
dc.date.accessioned
2017-12-14T09:40:13Z
dc.date.available
2017-11-22T08:38:18Z
dc.date.available
2017-12-14T09:40:13Z
dc.date.issued
2017-09-29
dc.identifier.issn
0021-9673
dc.identifier.other
10.1016/j.chroma.2017.08.013
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/211860
dc.identifier.doi
10.3929/ethz-b-000211860
dc.description.abstract
Mutarotation of sugars causes distorted and splitted peaks in liquid chromatography. The shape of the elution profile is affected by the different anomeric and isomeric forms in solution. The rate of interconversion between the different forms relative to the propagation velocity in the column determines the extent of the distortion. This interplay of interconversion reaction and chromatographic separation was examined both experimentally and theoretically. Elution profiles resulting from pulse injections of glucose and fructose solutions at different flow rates and temperatures were analyzed both qualitatively and quantitatively. Adsorption equilibrium and reaction kinetic parameters were estimated by a simple fitting procedure, based on peak elution times and area ratios obtained from the analysis of the experimental profiles. To enhance the accuracy of the model parameters further, estimated reaction kinetic parameters were provided as an initial guess for inverse fitting to elution profiles, using a numeric mass balance model. Simulations with the numeric model, based on the enhanced parameters, allowed a very precise description of the experimental profiles. Accuracy of the fitted parameters was further confirmed through comparison with literature values. Reaction rate constants for the adsorbed phase were calculated and interpreted based on apparent rate constant values determined in this work, and on literature data for aqueous solutions.
en_US
dc.format
application/pdf
dc.language.iso
en
en_US
dc.publisher
Elsevier
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Mutarotation
en_US
dc.subject
Sugar separations
en_US
dc.subject
Liquid chromatography
en_US
dc.subject
Mathematical modeling
en_US
dc.subject
Parameter estimation
en_US
dc.title
Interconversion and chromatographic separation of carbohydrate stereoisomers on polystyrene-divinylbenzene resins
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2017-09-29
ethz.journal.title
Journal of Chromatography A
ethz.journal.volume
1517
en_US
ethz.pages.start
54
en_US
ethz.pages.end
65
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Amsterdam
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02130 - Dep. Maschinenbau und Verfahrenstechnik / Dep. of Mechanical and Process Eng.::02668 - Inst. f. Energie- und Verfahrenstechnik / Inst. Energy and Process Engineering::03484 - Mazzotti, Marco / Mazzotti, Marco
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02130 - Dep. Maschinenbau und Verfahrenstechnik / Dep. of Mechanical and Process Eng.::02668 - Inst. f. Energie- und Verfahrenstechnik / Inst. Energy and Process Engineering::03484 - Mazzotti, Marco / Mazzotti, Marco
ethz.relation.isCitedBy
10.3929/ethz-b-000270250
ethz.date.deposited
2017-11-22T08:38:36Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-12-14T09:40:15Z
ethz.rosetta.lastUpdated
2021-02-14T21:22:53Z
ethz.rosetta.versionExported
true
ethz.COinS
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