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dc.contributor.author
Freimann, Remo
dc.contributor.author
Wutz, Anton
dc.date.accessioned
2017-12-14T09:35:46Z
dc.date.available
2017-11-22T08:42:27Z
dc.date.available
2017-12-14T09:35:46Z
dc.date.issued
2017-09
dc.identifier.other
10.1063/1.5006326
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/211869
dc.identifier.doi
10.3929/ethz-b-000211869
dc.description.abstract
Hemizygous mutations introduced in haploid genomes can directly expose a phenotype, thus facilitating gene function analysis and forward genetic screening. Recently, mammalian haploid cells could be derived from mouse, rat, monkey, and human embryos and have been applied to screens of cellular mechanisms including cell signaling, pathogen host factors, and developmental pathways. Notably, haploid cell cultures have an intrinsic tendency for diploidization and, thus, require periodic cell sorting. Here, we report a method for rapid purification of haploid mouse embryonic stem cells from mixed cell populations with high viability and yield. Our method uses membranes with micrometer pores for force-free separation and facilitates enrichment of haploid cells without flow cytometry. The separation method simplifies maintaining haploid cell cultures and has further applications in establishing haploid cell lines from embryos and isolating cell cycle phases of mammalian cells.
en_US
dc.format
application/pdf
dc.language.iso
en
en_US
dc.publisher
American Institute of Physics
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
A fast and efficient size separation method for haploid embryonic stem cells
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2017-10-31
ethz.journal.title
Biomicrofluidics
ethz.journal.volume
11
en_US
ethz.journal.issue
5
en_US
ethz.pages.start
054117
en_US
ethz.size
7 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
X chromosome reactivation in development and transformation
en_US
ethz.grant
Forward genetic screening in mouse haploid embryonic stem cells (ESCs) to functionally dissect pathways in mammalian development and disease
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
College Park, MD
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02539 - Institut für Molecular Health Sciences / Institute of Molecular Health Sciences::03978 - Wutz, Anton / Wutz, Anton
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02539 - Institut für Molecular Health Sciences / Institute of Molecular Health Sciences::03978 - Wutz, Anton / Wutz, Anton
ethz.grant.agreementno
152814
ethz.grant.agreementno
145026
ethz.grant.fundername
SNF
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Projektförderung in Biologie und Medizin (Abteilung III)
ethz.grant.program
R'Equip
ethz.date.deposited
2017-11-22T08:42:43Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-12-14T09:35:48Z
ethz.rosetta.lastUpdated
2021-02-14T21:22:51Z
ethz.rosetta.versionExported
true
ethz.COinS
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