Show simple item record

dc.contributor.author
Arnoldini, Simon
dc.contributor.author
Moscaroli, Alessandra
dc.contributor.author
Chabria, Mamta
dc.contributor.author
Hilbert, Manuel
dc.contributor.author
Hertig, Samuel
dc.contributor.author
Schibli, Roger
dc.contributor.author
Béhé, Martin
dc.contributor.author
Vogel, Viola
dc.date.accessioned
2017-11-29T11:39:47Z
dc.date.available
2017-11-27T11:50:01Z
dc.date.available
2017-11-29T11:39:47Z
dc.date.issued
2017-11-27
dc.identifier.other
10.1038/s41467-017-01846-0
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/213976
dc.identifier.doi
10.3929/ethz-b-000213976
dc.description.abstract
Transformations of extracellular matrix (ECM) accompany pathological tissue changes, yet how cell-ECM crosstalk drives these processes remains unknown as adequate tools to probe forces or mechanical strains in tissues are lacking. Here, we introduce a new nanoprobe to assess the mechanical strain of fibronectin (Fn) fibers in tissue, based on the bacterial Fn-binding peptide FnBPA5. FnBPA5 exhibits nM binding affinity to relaxed, but not stretched Fn fibers and is shown to exhibit strain-sensitive ECM binding in cell culture in a comparison with an established Fn-FRET probe. Staining of tumor tissue cryosections shows large regions of relaxed Fn fibers and injection of radiolabeled 111In-FnBPA5 in a prostate cancer mouse model reveals specific accumulation of 111In-FnBPA5 in tumor with prolonged retention compared to other organs. The herein presented approach enables to investigate how Fn fiber strain at the tissue level impacts cell signaling and pathological progression in different diseases.
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publications Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Cancer screening
en_US
dc.subject
Diagnostic markers
en_US
dc.subject
Sensors and probes
en_US
dc.subject
Tumour heterogeneity
en_US
dc.title
Novel peptide probes to assess the tensional state of fibronectin fibers in cancer
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
Nature Communications
ethz.journal.volume
8
en_US
ethz.journal.issue
1
en_US
ethz.pages.start
1793
en_US
ethz.size
13 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Mechanotransduction processes, from the cell periphery to the nucleus, in 2D versus 3D microenvironments
en_US
ethz.grant
Novel Imaging and Therapeutic Tools in Systemic Sclerosis
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02540 - Institut für Translationale Medizin / Institute of Translational Medicine::03640 - Vogel, Viola / Vogel, Viola
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02534 - Institut für Pharmazeutische Wiss. / Institute of Pharmaceutical Sciences::03688 - Schibli, Roger / Schibli, Roger
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02540 - Institut für Translationale Medizin / Institute of Translational Medicine::03640 - Vogel, Viola / Vogel, Viola
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02534 - Institut für Pharmazeutische Wiss. / Institute of Pharmaceutical Sciences::03688 - Schibli, Roger / Schibli, Roger
ethz.grant.agreementno
156931
ethz.grant.agreementno
154490
ethz.grant.fundername
SNF
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Interdisziplinäres Projekt
ethz.grant.program
Sinergia
ethz.date.deposited
2017-11-27T11:50:02Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-11-29T11:39:55Z
ethz.rosetta.lastUpdated
2019-02-02T13:45:38Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Novel%20peptide%20probes%20to%20assess%20the%20tensional%20state%20of%20fibronectin%20fibers%20in%20cancer&rft.jtitle=Nature%20Communications&rft.date=2017-11-27&rft.volume=8&rft.issue=1&rft.spage=1793&rft.au=Arnoldini,%20Simon&Moscaroli,%20Alessandra&Chabria,%20Mamta&Hilbert,%20Manuel&Hertig,%20Samuel&rft.genre=article&
 Search via SFX

Files in this item

Thumbnail

Publication type

Show simple item record