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dc.contributor.author
Gujas, Bojan
dc.contributor.author
Cruz, Tiago M.D.
dc.contributor.author
Kastanaki, Elizabeth
dc.contributor.author
Vermeer, Joop E.M.
dc.contributor.author
Munnik, Teun
dc.contributor.author
Rodriguez-Villalon, Antia
dc.date.accessioned
2017-11-28T10:36:04Z
dc.date.available
2017-11-28T10:36:04Z
dc.date.available
2017-11-28T10:34:14Z
dc.date.available
2017-11-13T13:12:50Z
dc.date.available
2017-10-19T02:15:57Z
dc.date.issued
2017-10-01
dc.identifier.other
10.1242/dev.155788
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/214921
dc.identifier.doi
10.3929/ethz-b-000207414
dc.description.abstract
The plant vascular network consists of specialized phloem and xylem elements that undergo two distinct morphogenetic developmental programs to become transport-functional units. Whereas vacuolar rupture is a determinant step in protoxylem differentiation, protophloem elements never form a big central vacuole. Here, we show that a genetic disturbance of phosphatidylinositol 4,5-bis-phosphate [PtdIns(4,5)P2] homeostasis rewires cell trafficking towards the vacuole in Arabidopsis thaliana roots. Consequently, an enhanced phosphoinositide-mediated vacuolar biogenesis correlates with premature programmed cell death (PCD) and secondary cell wall elaboration in xylem cells. By contrast, vacuolar fusion events in protophloem cells trigger the abnormal formation of big vacuoles, preventing cell clearance and tissue functionality. Removal of the inositol 5′ phosphatase COTYLEDON VASCULAR PATTERN 2 from the plasma membrane (PM) by brefeldin A (BFA) treatment increases PtdIns(4,5)P2 content at the PM and disrupts protophloem continuity. Conversely, BFA application abolishes vacuolar fusion events in xylem tissue without preventing PCD, suggesting the existence of additional PtdIns(4,5)P2-dependent cell death mechanisms. Overall, our data indicate that tight PM phosphoinositide homeostasis is required to modulate intracellular trafficking contributing to oppositely regulate vascular differentiation.
en_US
dc.language.iso
en
en_US
dc.publisher
Company of Biologists
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.subject
Phloem
en_US
dc.subject
Xylem
en_US
dc.subject
Vacuole
en_US
dc.subject
Intracellular trafficking
en_US
dc.subject
BFA
en_US
dc.subject
Programmed cell death
en_US
dc.title
Perturbing phosphoinositide homeostasis oppositely affects vascular differentiation in Arabidopsis thaliana roots.
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
Development
ethz.journal.volume
144
en_US
ethz.journal.issue
19
en_US
ethz.pages.start
3578
en_US
ethz.pages.end
3589
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Cambridge
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02541 - Institut für Molekulare Pflanzenbiologie / Institute of Molecular Plant Biology::09493 - Rodriguez-Villalon, Antia / Rodriguez-Villalon, Antia
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02541 - Institut für Molekulare Pflanzenbiologie / Institute of Molecular Plant Biology::09493 - Rodriguez-Villalon, Antia / Rodriguez-Villalon, Antia
en_US
ethz.date.deposited
2017-10-19T02:16:06Z
ethz.source
FORM
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-11-29T07:55:38Z
ethz.rosetta.lastUpdated
2018-11-06T04:20:33Z
ethz.rosetta.versionExported
true
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/207414
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/197776
ethz.COinS
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