Bakkum, Douglas J.
- Journal Article
Rights / licenseCreative Commons Attribution 4.0 International
Axons are neuronal processes specialized for conduction of action potentials (APs). The timing and temporal precision of APs when they reach each of the synapses are fundamentally important for information processing in the brain. Due to small diameters of axons, direct recording of single AP transmission is challenging. Consequently, most knowledge about axonal conductance derives from modeling studies or indirect measurements. We demonstrate a method to noninvasively and directly record individual APs propagating along millimeter-length axonal arbors in cortical cultures with hundreds of microelectrodes at microsecond temporal resolution. We find that cortical axons conduct single APs with high temporal precision (~100 µs arrival time jitter per mm length) and reliability: in more than 8,000,000 recorded APs, we did not observe any conduction or branch-point failures. Upon high-frequency stimulation at 100 Hz, successive became slower, and their arrival time precision decreased by 20% and 12% for the 100th AP, respectively Show more
Journal / serieseLife
Pages / Article No.
PublishereLife Sciences Publ.
Organisational unit03684 - Hierlemann, Andreas / Hierlemann, Andreas
157092 - Microtechnology and microelectronics to study mammalian axons (SNF)
132245 - Network dynamics underlying learning in embodied cortical brain cells grown over an 11,011-electrode CMOS circuit (SNF)
167989 - Position Dependence of Retinal Computation (SNF)
MoreShow all metadata