Unravelling Protein-Protein Interaction Networks Linked to Aliphatic and Indole Glucosinolate Biosynthetic Pathways in Arabidopsis

Open access
Author
Nintemann, Sebastian J.
Vik, Daniel
Svozil, Julia
Bak, Michael
Baerenfaller, Katja
Burow, Meike
Halkier, Barbara A.
Date
2017-11Type
- Journal Article
Abstract
Within the cell, biosynthetic pathways are embedded in protein-protein interaction networks. In Arabidopsis, the biosynthetic pathways of aliphatic and indole glucosinolate defense compounds are well-characterized. However, little is known about the spatial orchestration of these enzymes and their interplay with the cellular environment. To address these aspects, we applied two complementary, untargeted approaches—split-ubiquitin yeast 2-hybrid and co-immunoprecipitation screens—to identify proteins interacting with CYP83A1 and CYP83B1, two homologous enzymes specific for aliphatic and indole glucosinolate biosynthesis, respectively. Our analyses reveal distinct functional networks with substantial interconnection among the identified interactors for both pathway-specific markers, and add to our knowledge about how biochemical pathways are connected to cellular processes. Specifically, a group of protein interactors involved in cell death and the hypersensitive response provides a potential link between the glucosinolate defense compounds and defense against biotrophic pathogens, mediated by protein-protein interactions. Show more
Permanent link
https://doi.org/10.3929/ethz-b-000219944Publication status
publishedJournal / series
Frontiers in Plant ScienceVolume
Pages / Article No.
Publisher
Frontiers Research FoundationSubject
Arabidopsis thaliana; protein-protein interactions; glucosinolates; regulatory networks; pathway organizationMore
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