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dc.contributor.author
Kešnerová, Lucie
dc.contributor.author
Mars, Ruben A.T.
dc.contributor.author
Ellegaard, Kirsten M.
dc.contributor.author
Troilo, Michaël
dc.contributor.author
Sauer, Uwe
dc.contributor.author
Engel, Philipp
dc.date.accessioned
2018-01-19T12:40:59Z
dc.date.available
2017-12-28T03:40:23Z
dc.date.available
2018-01-19T12:40:59Z
dc.date.issued
2018
dc.identifier.issn
1544-9173
dc.identifier.issn
1545-7885
dc.identifier.other
10.1371/journal.pbio.2003467
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/224719
dc.identifier.doi
10.3929/ethz-b-000224719
dc.description.abstract
It is presently unclear how much individual community members contribute to the overall metabolic output of a gut microbiota. To address this question, we used the honey bee, which harbors a relatively simple and remarkably conserved gut microbiota with striking parallels to the mammalian system and importance for bee health. Using untargeted metabolomics, we profiled metabolic changes in gnotobiotic bees that were colonized with the complete microbiota reconstituted from cultured strains. We then determined the contribution of individual community members in mono-colonized bees and recapitulated our findings using in vitro cultures. Our results show that the honey bee gut microbiota utilizes a wide range of pollen-derived substrates, including flavonoids and outer pollen wall components, suggesting a key role for degradation of recalcitrant secondary plant metabolites and pollen digestion. In turn, multiple species were responsible for the accumulation of organic acids and aromatic compound degradation intermediates. Moreover, a specific gut symbiont, Bifidobacterium asteroides, stimulated the production of host hormones known to impact bee development. While we found evidence for cross-feeding interactions, approximately 80% of the identified metabolic changes were also observed in mono-colonized bees, with Lactobacilli being responsible for the largest share of the metabolic output. These results show that, despite prolonged evolutionary associations, honey bee gut bacteria can independently establish and metabolize a wide range of compounds in the gut. Our study reveals diverse bacterial functions that are likely to contribute to bee health and provide fundamental insights into how metabolic activities are partitioned within gut communities.
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Disentangling metabolic functions of bacteria in the honey bee gut
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2017-12-12
ethz.journal.title
PLoS Biology
ethz.journal.volume
15
en_US
ethz.journal.issue
12
en_US
ethz.journal.abbreviated
PLoS biol.
ethz.pages.start
e2003467
en_US
ethz.size
28 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.scopus
ethz.publication.place
Lawrence, KS
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03713 - Sauer, Uwe / Sauer, Uwe
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03713 - Sauer, Uwe / Sauer, Uwe
ethz.date.deposited
2017-12-28T03:40:25Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2018-01-19T12:41:06Z
ethz.rosetta.lastUpdated
2018-11-06T07:08:05Z
ethz.rosetta.versionExported
true
ethz.COinS
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