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dc.contributor.author
Berry, Michael H.
dc.contributor.author
Holt, Amy
dc.contributor.author
Levitz, Joshua
dc.contributor.author
Broichhagen, Johannes
dc.contributor.author
Gaub, Benjamin M.
dc.contributor.author
Visel, Meike
dc.contributor.author
Stanley, Cherise
dc.contributor.author
Aghi, Krishan
dc.contributor.author
Kim, Yang J.
dc.contributor.author
Cao, Kevin
dc.contributor.author
Kramer, Richard H.
dc.contributor.author
Trauner, Dirk
dc.contributor.author
Flannery, John
dc.contributor.author
Isacoff, Ehud Y.
dc.date.accessioned
2018-04-05T10:41:23Z
dc.date.available
2017-12-28T03:43:15Z
dc.date.available
2018-01-25T16:53:58Z
dc.date.available
2018-04-05T10:31:48Z
dc.date.available
2018-04-05T10:41:23Z
dc.date.issued
2017
dc.identifier.other
10.1038/s41467-017-01990-7
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/224747
dc.identifier.doi
10.3929/ethz-b-000224747
dc.description.abstract
Retinitis pigmentosa results in blindness due to degeneration of photoreceptors, but spares other retinal cells, leading to the hope that expression of light-activated signaling proteins in the surviving cells could restore vision. We used a retinal G protein-coupled receptor, mGluR2, which we chemically engineered to respond to light. In retinal ganglion cells (RGCs) of blind rd1 mice, photoswitch-charged mGluR2 (“SNAG-mGluR2”) evoked robust OFF responses to light, but not in wild-type retinas, revealing selectivity for RGCs that have lost photoreceptor input. SNAG-mGluR2 enabled animals to discriminate parallel from perpendicular lines and parallel lines at varying spacing. Simultaneous viral delivery of the inhibitory SNAG-mGluR2 and excitatory light-activated ionotropic glutamate receptor LiGluR yielded a distribution of expression ratios, restoration of ON, OFF and ON-OFF light responses and improved visual acuity. Thus, SNAG-mGluR2 restores patterned vision and combinatorial light response diversity provides a new logic for enhanced-acuity retinal prosthetics.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Molecular neuroscience
en_US
dc.subject
Protein design
en_US
dc.subject
Retina
en_US
dc.title
Restoration of patterned vision with an engineered photoactivatable G protein-coupled receptor
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2017-11-30
ethz.journal.title
Nature Communications
ethz.journal.volume
8
en_US
ethz.journal.issue
1
en_US
ethz.pages.start
1862
en_US
ethz.size
12 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.relation.isReferencedBy
10.1038/s41467-018-03585-2
ethz.date.deposited
2017-12-28T03:43:27Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2018-01-25T16:54:04Z
ethz.rosetta.lastUpdated
2018-02-01T11:09:17Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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