Show simple item record

dc.contributor.author
Basler, Lisa
dc.contributor.author
Gerdes, Stephan
dc.contributor.author
Wolfer, David P.
dc.contributor.author
Slomianka, Lutz
dc.date.accessioned
2018-01-15T14:05:20Z
dc.date.available
2018-01-03T02:29:19Z
dc.date.available
2018-01-15T14:05:20Z
dc.date.issued
2017-12
dc.identifier.issn
1662-5129
dc.identifier.other
10.3389/fnana.2017.00123
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/224968
dc.identifier.doi
10.3929/ethz-b-000224968
dc.description.abstract
Sampling is a critical step in procedures that generate quantitative morphological data in the neurosciences. Samples need to be representative to allow statistical evaluations, and samples need to deliver a precision that makes statistical evaluations not only possible but also meaningful. Sampling generated variability should, e.g., not be able to hide significant group differences from statistical detection if they are present. Estimators of the coefficient of error (CE) have been developed to provide tentative answers to the question if sampling has been “good enough” to provide meaningful statistical outcomes. We tested the performance of the commonly used Gundersen-Jensen CE estimator, using the layers of the mouse hippocampal dentate gyrus as an example (molecular layer, granule cell layer and hilus). We found that this estimator provided useful estimates of the precision that can be expected from samples of different sizes. For all layers, we found that a smoothness factor (m) of 0 generally provided better estimates than an m of 1. Only for the combined layers, i.e., the entire dentate gyrus, better CE estimates could be obtained using an m of 1. The orientation of the sections impacted on CE sizes. Frontal (coronal) sections are typically most efficient by providing the smallest CEs for a given amount of work. Applying the estimator to 3D-reconstructed layers and using very intense sampling, we observed CE size plots with m = 0 to m = 1 transitions that should also be expected but are not often observed in real section series. The data we present also allows the reader to approximate the sampling intervals in frontal, horizontal or sagittal sections that provide CEs of specified sizes for the layers of the mouse dentate gyrus.
en_US
dc.language.iso
en
en_US
dc.publisher
Frontiers Research Foundation
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
dentate gyrus
en_US
dc.subject
stereology
en_US
dc.subject
volume
en_US
dc.subject
Cavalieri estimator
en_US
dc.subject
CE estimators
en_US
dc.subject
C57BL/6 mice
en_US
dc.title
Sampling the Mouse Hippocampal Dentate Gyrus
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2017-12-12
ethz.journal.title
Frontiers in Neuroanatomy
ethz.journal.volume
11
en_US
ethz.journal.abbreviated
Front. neuroanat.
ethz.pages.start
123
en_US
ethz.size
10 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.publication.place
Lausanne
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02535 - Institut für Bewegungswiss. und Sport / Institut of Human Movement Sc. and Sport::03727 - Wolfer, David P. / Wolfer, David P.
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02535 - Institut für Bewegungswiss. und Sport / Institut of Human Movement Sc. and Sport::03727 - Wolfer, David P. / Wolfer, David P.
ethz.date.deposited
2018-01-03T02:29:54Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2018-01-15T14:05:28Z
ethz.rosetta.lastUpdated
2018-11-06T06:43:52Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Sampling%20the%20Mouse%20Hippocampal%20Dentate%20Gyrus&rft.jtitle=Frontiers%20in%20Neuroanatomy&rft.date=2017-12&rft.volume=11&rft.spage=123&rft.issn=1662-5129&rft.au=Basler,%20Lisa&Gerdes,%20Stephan&Wolfer,%20David%20P.&Slomianka,%20Lutz&rft.genre=article&
 Search via SFX

Files in this item

Thumbnail

Publication type

Show simple item record