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dc.contributor.author
Tecon, Robin
dc.contributor.author
Or, Dani
dc.date.accessioned
2018-01-08T07:30:03Z
dc.date.available
2018-01-05T13:58:13Z
dc.date.available
2018-01-08T07:30:03Z
dc.date.issued
2017-09-05
dc.identifier.uri
http://hdl.handle.net/20.500.11850/226019
dc.identifier.doi
10.3929/ethz-b-000226019
dc.description.abstract
Microbes are essential actors in all environments (nutrients cycling, pollutants bioremediation, and more), and their functions depend on multispecies communities that possess intricate interspecies interactions and high degree of spatial organization. Due to the complexity of natural microbial systems, mechanistic understanding of community spatial assembly and activity remains fragmentary. Here, we used a synthetic ecology approach to ask how simple factors such as carbon utilization control spatial patterns of a two-partner bacterial consortium grown on surfaces. The experimental model consortium consisted of two mutant strains of Pseudomonas putida that cooperate to degrade and utilize toluene. Strains tagging with different autofluorescent proteins allowed for microscopic visualization and pattern quantification by image analysis. We showed that trophic cooperation (toluene degradation) led to convergence of partner abundance (1:1) regardless of the initial ratio, and to strong strain intermixing at the microscale (10-100 m). In contrast, competition for a carbon source (benzoate) degraded independently by both strains resulted in distinct segregation patterns. The consortium productivity (cell growth) on toluene was affected by the initial partner ratio on surfaces but not in liquid cultures. This study pinpoints general principles of microbial community spatial organization with potential applications for natural and engineered microbial systems.
en_US
dc.format
PDF
en_US
dc.language.iso
en
en_US
dc.publisher
ETH Zurich
en_US
dc.rights.uri
http://rightsstatements.org/page/InC-NC/1.0/
dc.title
Cooperation controls partners spatial intermixing in a synthetic bacterial consortium
en_US
dc.type
Conference Poster
dc.rights.license
In Copyright - Non-Commercial Use Permitted
ethz.size
1 p.
en_US
ethz.event
3rd International SystemsX.ch Conference on Systems Biology 2017
en_US
ethz.event.location
Zurich, Switzerland
en_US
ethz.event.date
September 4-7, 2017
en_US
ethz.publication.place
Zurich
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02721 - Inst. f. Biogeochemie u. Schadstoffdyn. / Inst. Biogeochem. and Pollutant Dynamics::03812 - Or, Dani / Or, Dani
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02721 - Inst. f. Biogeochemie u. Schadstoffdyn. / Inst. Biogeochem. and Pollutant Dynamics::03812 - Or, Dani / Or, Dani
en_US
ethz.relation.references
10.3929/ethz-b-000129576
ethz.date.deposited
2018-01-05T13:58:13Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2018-01-08T07:30:11Z
ethz.rosetta.lastUpdated
2018-11-06T06:18:45Z
ethz.rosetta.versionExported
true
ethz.COinS
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