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dc.contributor.author
Vandoorne, Tijs
dc.contributor.author
De Smet, Stefan
dc.contributor.author
Ramaekers, Monique
dc.contributor.author
Van Thienen, Ruud
dc.contributor.author
De Bock, Katrien
dc.contributor.author
Clarke, Kieran
dc.contributor.author
Hespel, Peter J.L.
dc.date.accessioned
2018-01-11T07:24:22Z
dc.date.available
2017-08-30T10:51:55Z
dc.date.available
2017-08-30T12:15:38Z
dc.date.available
2017-08-30T12:17:36Z
dc.date.available
2017-11-28T10:02:24Z
dc.date.available
2017-12-04T15:39:14Z
dc.date.available
2018-01-10T16:37:21Z
dc.date.available
2018-01-11T07:24:22Z
dc.date.issued
2017-05-23
dc.identifier.other
10.3389/fphys.2017.00310
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/227424
dc.identifier.doi
10.3929/ethz-b-000227424
dc.description.abstract
Purpose: Ketone bodies are energy substrates produced by the liver during prolonged fasting or low-carbohydrate diet. The ingestion of a ketone ester (KE) rapidly increases blood ketone levels independent of nutritional status. KE has recently been shown to improve exercise performance, but whether it can also promote post-exercise muscle protein or glycogen synthesis is unknown. Methods: Eight healthy trained males participated in a randomized double-blind placebo-controlled crossover study. In each session, subjects undertook a bout of intense one-leg glycogen-depleting exercise followed by a 5-h recovery period during which they ingested a protein/carbohydrate mixture. Additionally, subjects ingested a ketone ester (KE) or an isocaloric placebo (PL). Results: KE intake did not affect muscle glycogen resynthesis, but more rapidly lowered post-exercise AMPK phosphorylation and resulted in higher mTORC1 activation, as evidenced by the higher phosphorylation of its main downstream targets S6K1 and 4E-BP1. As enhanced mTORC1 activation following KE suggests higher protein synthesis rates, we used myogenic C2C12 cells to further confirm that ketone bodies increase both leucine-mediated mTORC1 activation and protein synthesis in muscle cells. Conclusion: Our results indicate that adding KE to a standard post-exercise recovery beverage enhances the post-exercise activation of mTORC1 but does not affect muscle glycogen resynthesis in young healthy volunteers. In vitro, we confirmed that ketone bodies potentiate the increase in mTORC1 activation and protein synthesis in leucine-stimulated myotubes. Whether, chronic oral KE intake during recovery from exercise can facilitate training-induced muscular adaptation and remodeling need to be further investigated.
en_US
dc.language.iso
en
en_US
dc.publisher
Frontiers Research Foundation
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Muscle
en_US
dc.subject
Human
en_US
dc.subject
C2C12 myotubes
en_US
dc.subject
Protein
en_US
dc.subject
Glycogen
en_US
dc.subject
mTORC1 signaling
en_US
dc.subject
Ketone
en_US
dc.subject
Exercise recovery
en_US
dc.title
Intake of a Ketone Ester Drink during Recovery from Exercise Promotes mTORC1 Signaling but Not Glycogen Resynthesis in Human Muscle
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2017-05-23
ethz.journal.title
Frontiers in Physiology
ethz.journal.volume
8
en_US
ethz.pages.start
310
en_US
ethz.size
12 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Lausanne
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02535 - Institut für Bewegungswiss. und Sport / Institut of Human Movement Sc. and Sport::09560 - De Bock, Katrien / De Bock, Katrien
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02535 - Institut für Bewegungswiss. und Sport / Institut of Human Movement Sc. and Sport::09560 - De Bock, Katrien / De Bock, Katrien
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02535 - Institut für Bewegungswiss. und Sport / Institut of Human Movement Sc. and Sport::09560 - De Bock, Katrien / De Bock, Katrien
ethz.date.deposited
2017-08-30T10:51:58Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2018-01-11T07:19:21Z
ethz.rosetta.lastUpdated
2018-11-06T06:31:31Z
ethz.rosetta.versionExported
true
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/181794
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/216890
ethz.COinS
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