Gahlon, Hailey L.
- Journal Article
Rights / licenseCreative Commons Attribution 4.0 International
Objective Oxygen scavenging systems are routinely used during single-molecule imaging experiments to improve fluorescent dye stability. Previous work has shown nuclease contamination in the commonly used oxygen scavenging systems. This study evaluates the potential for nuclease contamination in these oxygen scavenging systems. Results Linear and plasmid DNA was incubated with two different oxygen scavenging systems (1) protocatechuic acid (PCA)-protocatechuate-3,4-dioxygenase (PCD) and (2) glucose-coupled glucose oxidase/catalase (GODCAT). No nucleic acid degradation was observed on single and double-stranded linear DNA and plasmid DNA, indicating the absence of nuclease contamination in these oxygen scavenging systems. Show more
Journal / seriesBMC Research Notes
Pages / Article No.
SubjectSingle ‑molecule imaging; Oxygen scavenging system; Protocatechuate ‑3,4 ‑dioxygenase; Protocatechuic acid; Glucose oxidase; Catalase
Organisational unit00002 - ETH Zürich
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