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dc.contributor.author
Gahlon, Hailey L.
dc.contributor.author
Poudevigne-Durance, Paul
dc.contributor.author
Rueda, David
dc.date.accessioned
2018-03-06T12:20:51Z
dc.date.available
2018-01-15T10:37:11Z
dc.date.available
2018-03-05T16:18:49Z
dc.date.available
2018-03-06T12:18:54Z
dc.date.available
2018-03-06T12:20:51Z
dc.date.issued
2017-11-21
dc.identifier.issn
1756-0500
dc.identifier.other
10.1186/s13104-017-2929-6
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/228529
dc.identifier.doi
10.3929/ethz-b-000228529
dc.description.abstract
Objective Oxygen scavenging systems are routinely used during single-molecule imaging experiments to improve fluorescent dye stability. Previous work has shown nuclease contamination in the commonly used oxygen scavenging systems. This study evaluates the potential for nuclease contamination in these oxygen scavenging systems. Results Linear and plasmid DNA was incubated with two different oxygen scavenging systems (1) protocatechuic acid (PCA)-protocatechuate-3,4-dioxygenase (PCD) and (2) glucose-coupled glucose oxidase/catalase (GODCAT). No nucleic acid degradation was observed on single and double-stranded linear DNA and plasmid DNA, indicating the absence of nuclease contamination in these oxygen scavenging systems.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
BioMed Central
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Single ‑molecule imaging
en_US
dc.subject
Oxygen scavenging system
en_US
dc.subject
Protocatechuate ‑3,4 ‑dioxygenase
en_US
dc.subject
Protocatechuic acid
en_US
dc.subject
Glucose oxidase
en_US
dc.subject
Catalase
en_US
dc.title
Absence of nuclease activity in commonly used oxygen-scavenging systems
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
BMC Research Notes
ethz.journal.volume
10
en_US
ethz.journal.issue
1
en_US
ethz.pages.start
606
en_US
ethz.size
4 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
::ETH Zürich::00002 - ETH Zürich
en_US
ethz.date.deposited
2018-01-15T10:37:11Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2018-03-06T12:18:59Z
ethz.rosetta.lastUpdated
2018-11-06T14:45:57Z
ethz.rosetta.versionExported
true
ethz.COinS
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