Monitoring the response of single MCF-7 cells to anticancer peptides on a microfluidic chip
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We report a microfluidic chip that can capture and isolate up to 612 single mammalian cells to monitor the ef-fect of treatment with anticancer peptides Aurein 1.2 and Aurein 2.2. Therefore, single breast adenocarcinoma cells (MCF-7) were confined in microfluidic chambers with a volume of less than 100 pl. Doughnut-shaped valves enabled us to quickly exchange solutions on demand to expose single cells to the membranolytic anti-cancer peptide (ACP) of interest. Automated fluorescence microscopy is employed to monitor the heterogeneous cellular response in all 612 chambers at high temporal resolution. Show more
Book title21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017)
Pages / Article No.
PublisherChemical and Biological Microsystems Society
SubjectSingle-cell analysis; Drug response monitoring; Anticancer peptides; Microfluidics
Organisational unit02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.
03807 - Dittrich, Petra / Dittrich, Petra
681587 - Engineering of hybrid cells using lab-on-chip technology (EC)
157190 - Experiment-guided computational de novo exploration of peptide-membrane interaction (SNF)
160699 - Engineering the Targeted Drugs of the Future: A General Approach (SNF)
NotesConference lecture held on October 26, 2017.
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