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dc.contributor.author
Michel, Agnès H.
dc.contributor.author
Hatakeyama, Riko
dc.contributor.author
Kimmig, Philipp
dc.contributor.author
Arter, Meret
dc.contributor.author
Peter, Matthias
dc.contributor.author
Matos, Joao
dc.contributor.author
De Virgilio, Claudio
dc.contributor.author
Kornmann, Benoît
dc.date.accessioned
2018-01-15T11:45:09Z
dc.date.available
2017-10-06T02:30:18Z
dc.date.available
2017-11-07T15:17:34Z
dc.date.available
2018-01-10T13:28:01Z
dc.date.available
2018-01-15T11:45:09Z
dc.date.issued
2017-05-08
dc.identifier.other
10.7554/eLife.23570
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/229099
dc.identifier.doi
10.3929/ethz-b-000190804
dc.description.abstract
Yeast is a powerful model for systems genetics. We present a versatile, time- and labor-efficient method to functionally explore the Saccharomyces cerevisiae genome using saturated transposon mutagenesis coupled to high-throughput sequencing. SAturated Transposon Analysis in Yeast (SATAY) allows one-step mapping of all genetic loci in which transposons can insert without disrupting essential functions. SATAY is particularly suited to discover loci important for growth under various conditions. SATAY (1) reveals positive and negative genetic interactions in single and multiple mutant strains, (2) can identify drug targets, (3) detects not only essential genes, but also essential protein domains, (4) generates both null and other informative alleles. In a SATAY screen for rapamycin-resistant mutants, we identify Pib2 (PhosphoInositide-Binding 2) as a master regulator of TORC1. We describe two antagonistic TORC1-activating and -inhibiting activities located on opposite ends of Pib2. Thus, SATAY allows to easily explore the yeast genome at unprecedented resolution and throughput.
en_US
dc.language.iso
en
en_US
dc.publisher
eLife Sciences Publ.
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Functional mapping of yeast genomes by saturated transposition
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
eLife
ethz.journal.volume
6
en_US
ethz.pages.start
e23570
en_US
ethz.size
28 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Molecular basis of interorganelle communication
en_US
ethz.grant
Coordination of DNA repair and segregation during meiosis and mitosis
en_US
ethz.grant
Rewiring the DNA repair machinery for genome stability and haploidisation
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Cambridge
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03595 - Peter, Matthias / Peter, Matthias
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03923 - Kornmann, Benoît (SNF-Professur) (ehem.) / Kornmann, Benoît (SNF-Professur) (form.)
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03595 - Peter, Matthias / Peter, Matthias
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::09457 - Matos, Joao / Matos, Joao
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03595 - Peter, Matthias / Peter, Matthias
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03923 - Kornmann, Benoît (SNF-Professur) (ehem.) / Kornmann, Benoît (SNF-Professur) (form.)
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03595 - Peter, Matthias / Peter, Matthias
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::09457 - Matos, Joao / Matos, Joao
en_US
ethz.grant.agreementno
133651
ethz.grant.agreementno
153058
ethz.grant.agreementno
155823
ethz.grant.fundername
SNF
ethz.grant.fundername
SNF
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
SNF-Förderungsprofessuren Stufe 2
ethz.grant.program
Projektförderung in Biologie und Medizin (Abteilung III)
ethz.grant.program
ethz.date.deposited
2017-10-06T02:30:35Z
ethz.source
WOS
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2018-01-16T08:08:09Z
ethz.rosetta.lastUpdated
2018-11-06T06:46:21Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/227199
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/190804
ethz.COinS
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