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dc.contributor.author
Schaber, Joerg
dc.contributor.author
Angel Adrover, Miquel
dc.contributor.author
Eriksson, Emma
dc.contributor.author
Pelet, Serge
dc.contributor.author
Petelenz-Kurdziel, Elzbieta
dc.contributor.author
Klein, Dagmara
dc.contributor.author
Posas, Francesc
dc.contributor.author
Goksor, Mattias
dc.contributor.author
Peter, Mathias
dc.contributor.author
Hohmann, Stefan
dc.contributor.author
Klipp, Edda
dc.date.accessioned
2023-09-22T14:14:37Z
dc.date.available
2017-06-09T05:39:19Z
dc.date.available
2023-09-22T14:14:37Z
dc.date.issued
2010-10
dc.identifier.issn
0175-7571
dc.identifier.issn
1432-1017
dc.identifier.other
10.1007/s00249-010-0612-0
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/23105
dc.identifier.doi
10.3929/ethz-b-000023105
dc.description.abstract
Parameterized models of biophysical and mechanical cell properties are important for predictive mathematical modeling of cellular processes. The concepts of turgor, cell wall elasticity, osmotically active volume, and intracellular osmolarity have been investigated for decades, but a consistent rigorous parameterization of these concepts is lacking. Here, we subjected several data sets of minimum volume measurements in yeast obtained after hyper-osmotic shock to a thermodynamic modeling framework. We estimated parameters for several relevant biophysical cell properties and tested alternative hypotheses about these concepts using a model discrimination approach. In accordance with previous reports, we estimated an average initial turgor of 0.6 ± 0.2 MPa and found that turgor becomes negligible at a relative volume of 93.3 ± 6.3% corresponding to an osmotic shock of 0.4 ± 0.2 Osm/l. At high stress levels (4 Osm/l), plasmolysis may occur. We found that the volumetric elastic modulus, a measure of cell wall elasticity, is 14.3 ± 10.4 MPa. Our model discrimination analysis suggests that other thermodynamic quantities affecting the intracellular water potential, for example the matrix potential, can be neglected under physiological conditions. The parameterized turgor models showed that activation of the osmosensing high osmolarity glycerol (HOG) signaling pathway correlates with turgor loss in a 1:1 relationship. This finding suggests that mechanical properties of the membrane trigger HOG pathway activation, which can be represented and quantitatively modeled by turgor.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Springer
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc/2.0/
dc.subject
Turgor
en_US
dc.subject
Cell wall elasticity
en_US
dc.subject
Volumetric elastic modulus
en_US
dc.subject
High osmolarity glycerol (HOG) signaling
en_US
dc.subject
Plasmolysis
en_US
dc.subject
Model discrimination
en_US
dc.subject
Yeast
en_US
dc.title
Biophysical properties of Saccharomyces cerevisiae and their relationship with HOG pathway activation
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial 2.0 Generic
dc.date.published
2010-06-19
ethz.journal.title
European Biophysics Journal
ethz.journal.volume
39
en_US
ethz.journal.issue
11
en_US
ethz.journal.abbreviated
Eur Biophys J
ethz.pages.start
1547
en_US
ethz.pages.end
1556
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
001747689
ethz.publication.place
Heidelberg
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03595 - Peter, Matthias / Peter, Matthias
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03595 - Peter, Matthias / Peter, Matthias
ethz.date.deposited
2017-06-09T05:39:28Z
ethz.source
ECIT
ethz.identifier.importid
imp59364d0c6a7a561178
ethz.ecitpid
pub:38081
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-12T11:51:41Z
ethz.rosetta.lastUpdated
2024-02-03T03:59:21Z
ethz.rosetta.versionExported
true
ethz.COinS
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