A microfluidic tilting platform for in vivo-like drug exposure of three-dimensional microtissues

Abstract

We present a microfluidic tilting platform with gravity-driven flow that enables culturing of three-dimensional microtissues (MTs) under in vivo-like drug dosing regimens. In contrast to traditional, constant dosing regimens, the chip allows for gradual changes in substance concentrations. Changes in the chip operation parameters, such as the use of different tilting angles, enable to alter the drug dosage on demand. In vivo concentration curves of acetaminophen (APAP) could be approximated with the device, while feasibility experiments using preformed MTs confirmed the chip’s biocompatibility.