Integrating multi-electrode arrays in microfluidic hanging-drop networks

Abstract

This work presents a highly versatile and scalable analytical platform for 3D microtissues. The device combines microfluidics for precise control of culture conditions with sensor technology for parallel and real-time monitoring of microtissue metabolism. The technology is based on an inverted, open microfluidic network. Interconnected hanging drops are formed underneath a patterned glass chip, which hosts the integrated microelectrode array. The electrodes can be individually functionalized and used as biosensors. The fabrication is simple, and the overall system can be expanded through the integration of larger numbers of hanging drops and more electrodes. The device can be used as a microfluidic platform for cultivation and continuous monitoring of 3D microtissues.