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dc.contributor.author
Gecaj, Rreze M.
dc.contributor.author
Schanzenbach, Corina I.
dc.contributor.author
Kirchner, Benedikt
dc.contributor.author
Pfaffl, Michael W.
dc.contributor.author
Riedmaier, Irmgard
dc.contributor.author
Tweedie-Cullen, Ry Y.
dc.contributor.author
Berisha, Bajram
dc.date.accessioned
2018-01-25T11:20:22Z
dc.date.available
2018-01-25T11:18:44Z
dc.date.available
2018-01-25T11:11:40Z
dc.date.available
2018-01-23T17:02:25Z
dc.date.available
2017-12-28T03:36:52Z
dc.date.available
2018-01-25T11:20:22Z
dc.date.issued
2017-12
dc.identifier.issn
1664-8021
dc.identifier.other
10.3389/fgene.2017.00213
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/237770
dc.identifier.doi
10.3929/ethz-b-000224652
dc.description.abstract
The formation, function, and subsequent regression of the ovarian corpus luteum (CL) are dynamic processes that enable ovary cyclical activity. Studies in whole ovary tissue have found microRNAs (miRNAs) to by critical for ovary function. However, relatively little is known about the role of miRNAs in the bovine CL. Utilizing small RNA next-generation sequencing we profiled miRNA transcriptome in bovine CL during the entire physiological estrous cycle, by sampling the CL on days: d 1–2, d 3–4, and d 5–7 (early CL, eCL), d 8–12 (mid CL, mCL), d 13–16 (late CL, lCL), and d > 18 (regressed CL, rCL). We characterized patterns of miRNAs abundance and identified 42 miRNAs that were consistent significantly different expressed (DE) in the eCL relative to their expression at each of the analyzed stages (mCL, lCL, and rCL). Out of these, bta-miR-210-3p, −2898, −96, −7-5p, −183-5p, −182, and −202 showed drastic up-regulation with a fold-change of ≥2.0 and adjusted P < 0.01 in the eCL, while bta-miR-146a was downregulated at lCL and rCL vs. the eCL. Another 24, 11, and 21 miRNAs were significantly DE only between individual comparisons, eCL vs. the mCL, lCL, and rCL, respectively. Irrespective of cycle stage two miRNAs, bta-miR-21-5p and bta-miR-143 were identified as the most abundant miRNAs species and show opposing expression abundance. Whilst bta-miR-21-5p peaked in number of reads in the eCL and was significantly downregulated in the mCL and lCL, bta-miR-143 reached its peak in the rCL and is significantly downregulated in the eCL. MiRNAs with significant DE in at least one cycle stage (CL class) were further grouped into eight distinct clusters by the self-organizing tree algorithm (SOTA). Half of the clusters contain miRNAs with low-expression, whilst the other half contain miRNAs with high-expression levels during eCL. Prediction analysis for significantly DE miRNAs resulted in target genes involved with CL formation, functionalization and CL regression. This study is the most comprehensive profiling of miRNA transcriptome in bovine CL covering the entire estrous cycle and provides a compact database for further functional validation and biomarker identification relevant for CL viability and fertility.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Frontiers Research Foundation
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
microRNA
en_US
dc.subject
small RNA Seq
en_US
dc.subject
bovine
en_US
dc.subject
corpus luteum
en_US
dc.subject
estrous cycle
en_US
dc.subject
SOTA
en_US
dc.title
The Dynamics of microRNA Transcriptome in Bovine Corpus Luteum during Its Formation, Function, and Regression
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2017-12-15
ethz.journal.title
Frontiers in Genetics
ethz.journal.volume
8
en_US
ethz.journal.abbreviated
Front. genet.
ethz.pages.start
213
en_US
ethz.size
16 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Lausanne
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03999 - Ulbrich, Susanne / Ulbrich, Susanne
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03999 - Ulbrich, Susanne / Ulbrich, Susanne
en_US
ethz.date.deposited
2017-12-28T03:36:59Z
ethz.source
FORM
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2018-01-31T14:57:35Z
ethz.rosetta.lastUpdated
2018-11-06T07:46:15Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/233363
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/224652
ethz.COinS
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