Dicer, a new regulator of pluripotency exit and LINE-1 elements in mouse embryonic stem cells
Ngondo, Richard P.
- Journal Article
Rights / licenseCreative Commons Attribution 4.0 International
gene regulation network orchestrates processes ensuring the maintenance of cellular identity and genome integrity. Small RNAs generated by the RNAse III DICER have emerged as central players in this network. Moreover, deletion of Dicer in mice leads to early embryonic lethality. To better understand the underlying mechanisms leading to this phenotype, we generated Dicer-deficient mouse embryonic stem cells (mESCs). Their detailed characterization revealed an impaired differentiation potential, and incapacity to exit from the pluripotency state. We also observed a strong accumulation of LINE-1 (L1s) transcripts, which was translated at protein level and led to an increased L1s retrotransposition. Our findings reveal Dicer as a new essential player that sustains mESCs self-renewal and genome integrity by controlling L1s regulation. Show more
Journal / seriesFEBS Open Bio
Pages / Article No.
SubjectDicer; LINE-1 retrotransposition; mouse embryonic stem cells; transposable elements
Organisational unit03983 - Ciaudo, Constance / Ciaudo, Constance
153220 - Nuclear role of the Argonaute proteins in mammalian embryonic stem cells (SNF)
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