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dc.contributor.author
Javed, Aneela
dc.contributor.author
Leuchte, Nicole
dc.contributor.author
Salinas, Gabriela
dc.contributor.author
Opitz, Lennart
dc.contributor.author
Stahl-Hennig, Christiane
dc.contributor.author
Sopper, Sieghart
dc.contributor.author
Sauermann, Ulrike
dc.date.accessioned
2018-02-20T16:31:06Z
dc.date.available
2018-02-16T09:47:29Z
dc.date.available
2018-02-16T09:45:19Z
dc.date.available
2017-06-12T18:35:00Z
dc.date.available
2018-02-16T09:46:08Z
dc.date.available
2018-01-18T14:07:41Z
dc.date.available
2018-02-20T16:31:06Z
dc.date.issued
2016-12
dc.identifier.other
10.1099/jgv.0.000632
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/242318
dc.identifier.doi
10.3929/ethz-b-000242318
dc.description.abstract
CD8+ cells from simian immunodeficiency virus (SIV)-infected long-term non-progressors and some uninfected macaques can suppress viral replication in vitro without killing the infected cells. The aim of this study was to identify factors responsible for non-cytolytic viral suppression by transcriptional profiling and to investigate their potential impact on SIV replication. Results of microarray experiments and further validation with cells from infected and uninfected macaques revealed that FAM26F RNA levels distinguished CD8+ cells of controllers and non-controllers (P=0.001). However, FAM26F was also expressed in CD4+ T-cells and B-cells. FAM26F expression increased in lymphocytes after in vitro IFN-gamma treatment on average 40-fold, and ex vivo FAM26F RNA levels in peripheral blood mononuclear cells correlated with plasma IFN-gamma but not with IFN-alpha. Baseline FAM26F expression appeared to be stable for months, albeit the individual expression levels varied up to tenfold. Investigating its role in SIV-infection revealed that FAM26F was upregulated after infection (P<0.0008), but did not directly correlate with viral load in contrast to MX1 and CXCL10. However, pre-infection levels of FAM26F correlated inversely with overall plasma viral load (AUC) during the acute and post-acute phases of infection (e.g. AUC weeks post infection 0-8; no AIDS vaccine: P<0.0001, Spearman rank correlation coefficient (rs)=-0.89, n=16; immunized with an AIDS vaccine: P=0.033, rs=-0.43; n=25). FAM26F transcript levels prior to infection can provide information about the pace and strength of the antiviral immune response during the early stage of infection. FAM26F expression represented, in our experiments, one of the earliest prognostic markers, and could supplement major histocompatibility complex (MHC)-typing to predict disease progression before SIV-infection.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Society for General Microbiology
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Innate immune response
en_US
dc.subject
HIV
en_US
dc.subject
acute infection
en_US
dc.subject
SIV
en_US
dc.subject
IFN-gamma
en_US
dc.subject
FAM26F
en_US
dc.title
Pre-infection transcript levels of FAM26F in peripheral blood mononuclear cells inform about overall plasma viral load in acute and post-acute phase after simian immunodeficiency virus infection
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2016-12-16
ethz.journal.title
Journal of General Virology
ethz.journal.volume
97
en_US
ethz.journal.issue
12
en_US
ethz.pages.start
3400
en_US
ethz.pages.end
3412
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
000049353
ethz.publication.place
Reading
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung / Domain VP Research::02207 - Functional Genomics Center Zurich / Functional Genomics Center Zurich
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung / Domain VP Research::02207 - Functional Genomics Center Zurich / Functional Genomics Center Zurich
ethz.date.deposited
2017-06-12T18:35:25Z
ethz.source
ECIT
ethz.source
BATCH
ethz.identifier.importid
imp593655235b35461167
ethz.ecitpid
pub:189401
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2018-02-20T11:33:28Z
ethz.rosetta.lastUpdated
2020-02-15T11:21:48Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/126635
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/230911
ethz.COinS
ctx_ver=Z39.88-2004&amp;rft_val_fmt=info:ofi/fmt:kev:mtx:journal&amp;rft.atitle=Pre-infection%20transcript%20levels%20of%20FAM26F%20in%20peripheral%20blood%20mononuclear%20cells%20inform%20about%20overall%20plasma%20viral%20load%20in%20acute%20and%2&amp;rft.jtitle=Journal%20of%20General%20Virology&amp;rft.date=2016-12&amp;rft.volume=97&amp;rft.issue=12&amp;rft.spage=3400&amp;rft.epage=3412&amp;rft.au=Javed,%20Aneela&amp;Leuchte,%20Nicole&amp;Salinas,%20Gabriela&amp;Opitz,%20Lennart&amp;Stahl-Hennig,%20Christiane&amp;rft.genre=article&amp;
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