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dc.contributor.author
Zündel, Manuel
dc.contributor.author
Ehret, Alexander E.
dc.contributor.author
Mazza, Edoardo
dc.date.accessioned
2018-02-20T13:52:37Z
dc.date.available
2017-06-12T20:09:19Z
dc.date.available
2017-07-20T09:38:07Z
dc.date.available
2018-01-03T09:31:38Z
dc.date.available
2018-02-20T13:52:37Z
dc.date.issued
2017-02-24
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0172927
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/242753
dc.identifier.doi
10.3929/ethz-b-000129128
dc.description.abstract
Methods summarized by the term Traction Force Microscopy are widely used to quantify cellular forces in mechanobiological studies. These methods are inverse, in the sense that forces must be determined such that they comply with a measured displacement field. This study investigates how several experimental and analytical factors, originating in the realization of the experiments and the procedures for the analysis, affect the determined traction forces. The present results demonstrate that even for very high resolution measurements free of noise, traction forces can be significantly underestimated, while traction peaks are typically overestimated by 50% or more, even in the noise free case. Compared to this errors, which are inherent to the nature of the mechanical problem and its discretization, the effect of ignoring the out-of-plane displacement component, the interpolation scheme used between the discrete measurement points and the disregard of the geometrical non-linearities when using a nearly linear substrate material are less consequential. Nevertheless, a nonlinear elastic substrate, with strain-stiffening response and some degree of compressibility, can substantially improve the robustness of the reconstruction of traction forces over a wide range of magnitudes. This poses the need for a correct mechanical representation of these non-linearities during the traction reconstruction and a correct mechanical characterization of the substrate itself, especially for the large strain shear domain which is shown to plays a major role in the deformations induced by cells.
en_US
dc.format
application/pdf
dc.language.iso
en
en_US
dc.publisher
Public Library of Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Factors influencing the determination of cell traction forces
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2017-02-24
ethz.journal.title
PLoS ONE
ethz.journal.volume
12
en_US
ethz.journal.issue
2
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e0172927
en_US
ethz.size
18 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Mechanical biocompatibility of electrospun scaffolds for intervertebral disc repair
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
010850889
ethz.publication.place
San Francisco, CA
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02130 - Dep. Maschinenbau und Verfahrenstechnik / Dep. of Mechanical and Process Eng.::02618 - Institut für Mechanische Systeme / Institute of Mechanical Systems::03605 - Mazza, Edoardo / Mazza, Edoardo
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02130 - Dep. Maschinenbau und Verfahrenstechnik / Dep. of Mechanical and Process Eng.::02618 - Institut für Mechanische Systeme / Institute of Mechanical Systems::03605 - Mazza, Edoardo / Mazza, Edoardo
en_US
ethz.grant.agreementno
155918
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Projektförderung in Mathematik, Natur- und Ingenieurwissenschaften (Abteilung II)
ethz.date.deposited
2018-02-21T12:38:12Z
ethz.source
ECIT
ethz.source
FORM
ethz.identifier.importid
imp5936554f7428438238
ethz.ecitpid
pub:192087
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2018-02-21T12:38:20Z
ethz.rosetta.lastUpdated
2021-02-14T22:28:38Z
ethz.rosetta.versionExported
true
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/225091
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/129128
ethz.COinS
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