Show simple item record

dc.contributor.author
Pott, Moritz
dc.contributor.author
Hayashi, Takahiro
dc.contributor.author
Mori, Takahiro
dc.contributor.author
Mittl, Peer R.E.
dc.contributor.author
Green, Anthony P.
dc.contributor.author
Hilvert, Donald
dc.date.accessioned
2021-03-01T13:10:12Z
dc.date.available
2018-02-22T04:20:57Z
dc.date.available
2018-04-05T10:26:17Z
dc.date.available
2021-03-01T13:10:12Z
dc.date.issued
2018-01-31
dc.identifier.issn
0002-7863
dc.identifier.issn
1520-5126
dc.identifier.other
10.1021/jacs.7b12621
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/242960
dc.description.abstract
Expanding the range of genetically encoded metal coordination environments accessible within tunable protein scaffolds presents excellent opportunities for the creation of metalloenzymes with augmented properties and novel activities. Here, we demonstrate that installation of a noncanonical Nδ-methyl histidine (NMH) as the proximal heme ligand in the oxygen binding protein myoglobin (Mb) leads to substantial increases in heme redox potential and promiscuous peroxidase activity. Structural characterization of this catalytically modified myoglobin variant (Mb NMH) revealed significant changes in the proximal pocket, including alterations to hydrogen-bonding interactions involving the prosthetic porphyrin cofactor. Further optimization of Mb NMH via a combination of rational modification and several rounds of laboratory evolution afforded efficient peroxidase biocatalysts within a globin fold, with activities comparable to those displayed by nature’s peroxidases.
en_US
dc.language.iso
en
en_US
dc.publisher
American Chemical Society
en_US
dc.title
A Noncanonical Proximal Heme Ligand Affords an Efficient Peroxidase in a Globin Fold
en_US
dc.type
Journal Article
dc.date.published
2018-01-08
ethz.journal.title
Journal of the American Chemical Society
ethz.journal.volume
140
en_US
ethz.journal.issue
4
en_US
ethz.journal.abbreviated
J. Am. Chem. Soc.
ethz.pages.start
1535
en_US
ethz.pages.end
1543
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Washington, DC
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02514 - Laboratorium für Organische Chemie / Laboratory of Organic Chemistry::03492 - Hilvert, Donald (emeritus) / Hilvert, Donald (emeritus)
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02514 - Laboratorium für Organische Chemie / Laboratory of Organic Chemistry::03492 - Hilvert, Donald (emeritus) / Hilvert, Donald (emeritus)
ethz.date.deposited
2018-02-22T04:21:01Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2018-04-05T10:26:21Z
ethz.rosetta.lastUpdated
2022-03-29T05:30:55Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=A%20Noncanonical%20Proximal%20Heme%20Ligand%20Affords%20an%20Efficient%20Peroxidase%20in%20a%20Globin%20Fold&rft.jtitle=Journal%20of%20the%20American%20Chemical%20Society&rft.date=2018-01-31&rft.volume=140&rft.issue=4&rft.spage=1535&rft.epage=1543&rft.issn=0002-7863&1520-5126&rft.au=Pott,%20Moritz&Hayashi,%20Takahiro&Mori,%20Takahiro&Mittl,%20Peer%20R.E.&Green,%20Anthony%20P.&rft.genre=article&rft_id=info:doi/10.1021/jacs.7b12621&
 Search print copy at ETH Library

Files in this item

FilesSizeFormatOpen in viewer

There are no files associated with this item.

Publication type

Show simple item record