Argonaute 2 Is Required for Extra-embryonic Endoderm Differentiation of Mouse Embryonic Stem Cells

Open access
Date
2018-02-13Type
- Journal Article
Citations
Cited 11 times in
Web of Science
Cited 12 times in
Scopus
ETH Bibliography
yes
Altmetrics
Abstract
In mouse, although four Argonaute (AGO) proteins with partly overlapping functions in small-RNA pathways exist, only Ago2 deficiency causes embryonic lethality. To investigate the role of AGO2 during mouse early development, we generated Ago2-deficient mouse embryonic stem cells (mESCs) and performed a detailed characterization of their differentiation potential. Ago2 disruption caused a global reduction of microRNAs, which resulted in the misregulation of only a limited number of transcripts. We demonstrated, both in vivo and in vitro, that AGO2 is dispensable for the embryonic germ-layer formation. However, Ago2-deficient mESCs showed a specific defect during conversion into extra-embryonic endoderm cells. We proved that this defect is cell autonomous and can be rescued by both a catalytically active and an inactive Ago2, but not by Ago2 deprived of its RNA binding capacity or by Ago1 overexpression. Overall, our results suggest a role for AGO2 in stem cell differentiation. Show more
Permanent link
https://doi.org/10.3929/ethz-b-000242978Publication status
publishedExternal links
Journal / series
Stem Cell ReportsVolume
Pages / Article No.
Publisher
ElsevierOrganisational unit
03983 - Ciaudo, C. (ehemalig) / Ciaudo, C. (former)
03983 - Ciaudo, C. (ehemalig) / Ciaudo, C. (former)
03983 - Ciaudo, C. (ehemalig) / Ciaudo, C. (former)
Funding
153220 - Nuclear role of the Argonaute proteins in mammalian embryonic stem cells (SNF)
More
Show all metadata
Citations
Cited 11 times in
Web of Science
Cited 12 times in
Scopus
ETH Bibliography
yes
Altmetrics