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dc.contributor.author
Caudron, Fabrice
dc.contributor.author
Denarier, Eric
dc.contributor.author
Thibout-Quintana, Jenny-Constanza
dc.contributor.author
Brocard, Jacques
dc.contributor.author
Andrieux, Annie
dc.contributor.author
Fourest-Lieuvin, Anne
dc.date.accessioned
2018-11-15T10:25:58Z
dc.date.available
2017-06-09T06:18:22Z
dc.date.available
2018-11-15T10:25:58Z
dc.date.issued
2010-10-21
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0013553
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/24430
dc.identifier.doi
10.3929/ethz-b-000024430
dc.description.abstract
Ser172 of β tubulin is an important residue that is mutated in a human brain disease and phosphorylated by the cyclin-dependent kinase Cdk1 in mammalian cells. To examine the role of this residue, we used the yeast S. cerevisiae as a model and produced two different mutations (S172A and S172E) of the conserved Ser172 in the yeast β tubulin Tub2p. The two mutants showed impaired cell growth on benomyl-containing medium and at cold temperatures, altered microtubule (MT) dynamics, and altered nucleus positioning and segregation. When cytoplasmic MT effectors Dyn1p or Kar9p were deleted in S172A and S172E mutants, cells were viable but presented increased ploidy. Furthermore, the two β tubulin mutations exhibited synthetic lethal interactions with Bik1p, Bim1p or Kar3p, which are effectors of cytoplasmic and spindle MTs. In the absence of Mad2p-dependent spindle checkpoint, both mutations are deleterious. These findings show the importance of Ser172 for the correct function of both cytoplasmic and spindle MTs and for normal cell division.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
PLOS
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.title
Mutation of Ser172 in Yeast β Tubulin Induces Defects in Microtubule Dynamics and Cell Division
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
PLoS ONE
ethz.journal.volume
5
en_US
ethz.journal.issue
10
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e13553
en_US
ethz.size
11 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.publication.place
San Francisco, CA
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03532 - Barral, Yves / Barral, Yves
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03532 - Barral, Yves / Barral, Yves
ethz.date.deposited
2017-06-09T06:18:47Z
ethz.source
ECIT
ethz.identifier.importid
imp59364d26411c913911
ethz.ecitpid
pub:39838
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-24T09:02:15Z
ethz.rosetta.lastUpdated
2024-02-02T06:37:11Z
ethz.rosetta.versionExported
true
ethz.COinS
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