Show simple item record

dc.contributor.author
Sonnleitner, Elisabeth
dc.contributor.author
Wulf, Alexander
dc.contributor.author
Campagne, Sébastien
dc.contributor.author
Pei, Xue-Yuan
dc.contributor.author
Wolfinger, Michael T.
dc.contributor.author
Forlani, Giada
dc.contributor.author
Prindl, Konstantin
dc.contributor.author
Abdou, Laetitia
dc.contributor.author
Resch, Armin
dc.contributor.author
Allain, Frédéric H.-T.
dc.contributor.author
Luisi, Ben F.
dc.contributor.author
Urlaub, Henning
dc.contributor.author
Bläsi, Udo
dc.date.accessioned
2018-04-05T12:52:55Z
dc.date.available
2018-03-08T02:57:33Z
dc.date.available
2018-04-05T12:52:55Z
dc.date.issued
2018-02-16
dc.identifier.issn
1362-4962
dc.identifier.issn
0301-5610
dc.identifier.other
10.1093/nar/gkx1245
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/247419
dc.identifier.doi
10.3929/ethz-b-000247419
dc.description.abstract
In Pseudomonas aeruginosa the RNA chaperone Hfq and the catabolite repression control protein (Crc) act as post-transcriptional regulators during carbon catabolite repression (CCR). In this regard Crc is required for full-fledged Hfq-mediated translational repression of catabolic genes. RNAseq based transcriptome analyses revealed a significant overlap between the Crc and Hfq regulons, which in conjunction with genetic data supported a concerted action of both proteins. Biochemical and biophysical approaches further suggest that Crc and Hfq form an assembly in the presence of RNAs containing A-rich motifs, and that Crc interacts with both, Hfq and RNA. Through these interactions, Crc enhances the stability of Hfq/Crc/RNA complexes, which can explain its facilitating role in Hfq-mediated translational repression. Hence, these studies revealed for the first time insights into how an interacting protein can modulate Hfq function. Moreover, Crc is shown to interfere with binding of a regulatory RNA to Hfq, which bears implications for riboregulation. These results are discussed in terms of a working model, wherein Crc prioritizes the function of Hfq toward utilization of favored carbon sources.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Oxford University Press
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Interplay between the catabolite repression control protein Crc, Hfq and RNA in Hfq-dependent translational regulation in Pseudomonas aeruginosa
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2017-12-13
ethz.journal.title
Nucleic Acids Research
ethz.journal.volume
46
en_US
ethz.journal.issue
3
en_US
ethz.journal.abbreviated
Nucleic Acids Res.
ethz.pages.start
1470
en_US
ethz.pages.end
1485
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Oxford
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03591 - Allain, Frédéric / Allain, Frédéric
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03591 - Allain, Frédéric / Allain, Frédéric
ethz.date.deposited
2018-03-08T02:58:25Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2018-04-05T12:52:58Z
ethz.rosetta.lastUpdated
2021-02-14T23:09:28Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Interplay%20between%20the%20catabolite%20repression%20control%20protein%20Crc,%20Hfq%20and%20RNA%20in%20Hfq-dependent%20translational%20regulation%20in%20Pseudomonas%20a&rft.jtitle=Nucleic%20Acids%20Research&rft.date=2018-02-16&rft.volume=46&rft.issue=3&rft.spage=1470&rft.epage=1485&rft.issn=1362-4962&0301-5610&rft.au=Sonnleitner,%20Elisabeth&Wulf,%20Alexander&Campagne,%20S%C3%A9bastien&Pei,%20Xue-Yuan&Wolfinger,%20Michael%20T.&rft.genre=article&rft_id=info:doi/10.1093/nar/gkx1245&
 Search print copy at ETH Library

Files in this item

Thumbnail

Publication type

Show simple item record