Show simple item record

dc.contributor.author
Passannante, Myriam
dc.contributor.author
Marti, Claude-Olivier
dc.contributor.author
Pfefferli, Catherine
dc.contributor.author
Moroni, Paolo S.
dc.contributor.author
Kaeser-Pebernard, Stéphanie
dc.contributor.author
Puoti, Alessandro
dc.contributor.author
Hunziker, Peter
dc.contributor.author
Wicky, Chantal
dc.contributor.author
Mueller, Fritz
dc.date.accessioned
2018-08-23T13:08:09Z
dc.date.available
2017-06-09T06:25:12Z
dc.date.available
2018-08-23T13:08:09Z
dc.date.issued
2010-10-27
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0013681
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/24756
dc.identifier.doi
10.3929/ethz-b-000024756
dc.description.abstract
Biochemical purifications from mammalian cells and Xenopus oocytes revealed that vertebrate Mi-2 proteins reside in multisubunit NuRD (Nucleosome Remodeling and Deacetylase) complexes. Since all NuRD subunits are highly conserved in the genomes of C. elegans and Drosophila, it was suggested that NuRD complexes also exist in invertebrates. Recently, a novel dMec complex, composed of dMi-2 and dMEP-1 was identified in Drosophila. The genome of C. elegans encodes two highly homologous Mi-2 orthologues, LET-418 and CHD-3. Here we demonstrate that these proteins define at least three different protein complexes, two distinct NuRD complexes and one MEC complex. The two canonical NuRD complexes share the same core subunits HDA-1/HDAC, LIN-53/RbAp and LIN-40/MTA, but differ in their Mi-2 orthologues LET-418 or CHD-3. LET-418 but not CHD-3, interacts with the Krüppel-like protein MEP-1 in a distinct complex, the MEC complex. Based on microarrays analyses, we propose that MEC constitutes an important LET-418 containing regulatory complex during C. elegans embryonic and early larval development. It is required for the repression of germline potential in somatic cells and acts when blastomeres are still dividing and differentiating. The two NuRD complexes may not be important for the early development, but may act later during postembryonic development. Altogether, our data suggest a considerable complexity in the composition, the developmental function and the tissue-specificity of the different C. elegans Mi-2 complexes.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.title
Different Mi-2 Complexes for Various Developmental Functions in Caenorhabditis elegans
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
PLoS ONE
ethz.journal.volume
5
en_US
ethz.journal.issue
10
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e13681
en_US
ethz.size
15 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
006206116
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung & Wirtschaftsbez. / Domain VP Research & Corporate Relations::02207 - Functional Genomics Center Zürich / Functional Genomics Center Zürich
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung & Wirtschaftsbez. / Domain VP Research & Corporate Relations::02207 - Functional Genomics Center Zürich / Functional Genomics Center Zürich
ethz.date.deposited
2017-06-09T06:25:27Z
ethz.source
ECIT
ethz.identifier.importid
imp59364d2c5bc5932169
ethz.ecitpid
pub:40269
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-19T08:19:24Z
ethz.rosetta.lastUpdated
2018-11-07T23:41:00Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Different%20Mi-2%20Complexes%20for%20Various%20Developmental%20Functions%20in%20Caenorhabditis%20elegans&rft.jtitle=PLoS%20ONE&rft.date=2010-10-27&rft.volume=5&rft.issue=10&rft.spage=e13681&rft.issn=1932-6203&rft.au=Passannante,%20Myriam&Marti,%20Claude-Olivier&Pfefferli,%20Catherine&Moroni,%20Paolo%20S.&Kaeser-Pebernard,%20St%C3%A9phanie&rft.genre=article&
 Search via SFX

Files in this item

Thumbnail

Publication type

Show simple item record