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dc.contributor.author
Zhou, Xuan
dc.contributor.author
Stevens, Marc J.A.
dc.contributor.author
Neuenschwander, Stefan
dc.contributor.author
Schwarm, Angela
dc.contributor.author
Kreuzer, Michael
dc.contributor.author
Bratus-Neuenschwander, Anna
dc.contributor.author
Zeitz, Johanna O.
dc.date.accessioned
2018-04-06T06:19:35Z
dc.date.available
2018-04-06T06:16:59Z
dc.date.available
2018-04-06T06:19:35Z
dc.date.issued
2018
dc.identifier.issn
1756-0500
dc.identifier.other
10.1186/s13104-018-3242-8
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/255738
dc.identifier.doi
10.3929/ethz-b-000244475
dc.description.abstract
Objective Lauric acid (C12) is a medium-chain fatty acid that inhibits growth and production of the greenhouse gas methane by rumen methanogens such as Methanobrevibacter ruminantium. To understand the inhibitory mechanism of C12, a transcriptome analysis was performed in M. ruminantium strain M1 (DSM 1093) using RNA-Seq. Results Pure cell cultures in the exponential growth phase were treated with 0.4 mg/ml C12, dissolved in dimethyl sulfoxide (DMSO), for 1 h and transcriptomic changes were compared to DMSO-only treated cells (final DMSO concentration 0.2%). Exposure to C12 resulted in differential expression of 163 of the 2280 genes in the M1 genome (maximum log2-fold change 6.6). Remarkably, C12 hardly affected the expression of genes involved in methanogenesis. Instead, most affected genes encode cell-surface associated proteins (adhesion-like proteins, membrane-associated transporters and hydrogenases), and proteins involved in detoxification or DNA-repair processes. Enrichment analysis on the genes regulated in the C12-treated group showed a significant enrichment for categories ‘cell surface’ and ‘mobile elements’ (activated by C12), and for the categories ‘regulation’ and ‘protein fate’ (represssed). These results are useful to generate and test specific hypotheses on the mechanism how C12 affects rumen methanogens.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
BioMed Central Ltd.
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Methanobrevibacter ruminantium
en_US
dc.subject
Methanogenesis
en_US
dc.subject
Fatty acid
en_US
dc.subject
Rumen
en_US
dc.subject
Gene expression
en_US
dc.subject
Lauric acid
en_US
dc.title
The transcriptome response of the ruminal methanogen Methanobrevibacter ruminantium strain M1 to the inhibitor lauric acid
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2018-02-17
ethz.journal.title
BMC Research Notes
ethz.journal.volume
11
en_US
ethz.journal.issue
135
en_US
ethz.pages.start
135
en_US
ethz.size
10 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.scopus
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02701 - Inst.f. Lebensmittelwiss.,Ernährung,Ges. / Institute of Food, Nutrition, and Health::03626 - Lacroix, Christophe / Lacroix, Christophe
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03428 - Kreuzer, Michael (emeritus) / Kreuzer, Michael (emeritus)
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung / Domain VP Research::02207 - Functional Genomics Center Zurich / Functional Genomics Center Zurich
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02701 - Inst.f. Lebensmittelwiss.,Ernährung,Ges. / Institute of Food, Nutrition, and Health::03626 - Lacroix, Christophe / Lacroix, Christophe
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03428 - Kreuzer, Michael (emeritus) / Kreuzer, Michael (emeritus)
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung / Domain VP Research::02207 - Functional Genomics Center Zurich / Functional Genomics Center Zurich
ethz.date.deposited
2018-02-20T15:01:30Z
ethz.source
SCOPUS
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2018-04-06T06:17:08Z
ethz.rosetta.lastUpdated
2021-02-14T23:10:24Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/244475
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/242519
ethz.COinS
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