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dc.contributor.author
Wojcik, Felix
dc.contributor.author
Dann, Geoffrey P.
dc.contributor.author
Beh, Leslie Y.
dc.contributor.author
Debelouchina, Galia T.
dc.contributor.author
Hofmann, Raphael
dc.contributor.author
Muir, Tom W.
dc.date.accessioned
2018-04-26T08:55:38Z
dc.date.available
2018-04-26T02:47:35Z
dc.date.available
2018-04-26T08:55:38Z
dc.date.issued
2018
dc.identifier.other
10.1038/s41467-018-03895-5
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/260647
dc.identifier.doi
10.3929/ethz-b-000260647
dc.description.abstract
Ubiquitylation of histone H2B at lysine residue 120 (H2BK120ub) is a prominent histone posttranslational modification (PTM) associated with the actively transcribed genome. Although H2BK120ub triggers several critical downstream histone modification pathways and changes in chromatin structure, less is known about the regulation of the ubiquitylation reaction itself, in particular with respect to the modification status of the chromatin substrate. Here we employ an unbiased library screening approach to profile the impact of pre-existing chromatin modifications on de novo ubiquitylation of H2BK120 by the cognate human E2:E3 ligase pair, UBE2A:RNF20/40. Deposition of H2BK120ub is found to be highly sensitive to PTMs on the N-terminal tail of histone H2A, a crosstalk that extends to the common histone variant H2A.Z. Based on a series of biochemical and cell-based studies, we propose that this crosstalk contributes to the spatial organization of H2BK120ub on gene bodies, and is thus important for transcriptional regulation.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Functional crosstalk between histone H2B ubiquitylation and H2A modifications and variants
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2018-04-11
ethz.journal.title
Nature Communications
ethz.journal.volume
9
en_US
ethz.pages.start
1394
en_US
ethz.size
11 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2018-04-26T02:48:18Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2018-04-26T08:55:49Z
ethz.rosetta.lastUpdated
2019-02-02T16:53:04Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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