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dc.contributor.author
Beck, Martin
dc.contributor.author
Schmidt, Alexander
dc.contributor.author
Malmström, Johan
dc.contributor.author
Claassen, Manfred
dc.contributor.author
Ori, Alessandro
dc.contributor.author
Szymborska, Anna
dc.contributor.author
Herzog, Franz
dc.contributor.author
Rinner, Oliver
dc.contributor.author
Ellenberg, Jan
dc.contributor.author
Aebersold, Ruedi
dc.date.accessioned
2018-06-08T06:22:46Z
dc.date.available
2018-06-08T06:22:46Z
dc.date.issued
2011-01
dc.identifier.other
10.1038/msb.2011.82
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/268325
dc.identifier.doi
10.3929/ethz-b-000041692
dc.description.abstract
The generation of mathematical models of biological processes, the simulation of these processes under different conditions, and the comparison and integration of multiple data sets are explicit goals of systems biology that require the knowledge of the absolute quantity of the system's components. To date, systematic estimates of cellular protein concentrations have been exceptionally scarce. Here, we provide a quantitative description of the proteome of a commonly used human cell line in two functional states, interphase and mitosis. We show that these human cultured cells express at least ∼10 000 proteins and that the quantified proteins span a concentration range of seven orders of magnitude up to 20 000 000 copies per cell. We discuss how protein abundance is linked to function and evolution.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publ. Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-sa/3.0/
dc.subject
Mass spectrometry
en_US
dc.subject
Protein abundance
en_US
dc.subject
Proteomics
en_US
dc.title
The quantitative proteome of a human cell line
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported
dc.date.published
2011-11-08
ethz.journal.title
Molecular Systems Biology
ethz.journal.volume
7
en_US
ethz.journal.issue
1
en_US
ethz.pages.start
549
en_US
ethz.size
8 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
004931271
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03663 - Aebersold, Rudolf / Aebersold, Rudolf
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03663 - Aebersold, Rudolf / Aebersold, Rudolf
ethz.date.deposited
2017-06-09T17:22:45Z
ethz.source
ECIT
ethz.identifier.importid
imp59364eac000c398789
ethz.identifier.importid
imp59364eaec81f115598
ethz.ecitpid
pub:69633
ethz.ecitpid
pub:69927
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2018-06-08T06:22:50Z
ethz.rosetta.lastUpdated
2018-11-07T11:32:18Z
ethz.rosetta.versionExported
true
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/162910
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/41692
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=The%20quantitative%20proteome%20of%20a%20human%20cell%20line&rft.jtitle=Molecular%20Systems%20Biology&rft.date=2011-01&rft.volume=7&rft.issue=1&rft.spage=549&rft.au=Beck,%20Martin&Schmidt,%20Alexander&Malmstr%C3%B6m,%20Johan&Claassen,%20Manfred&Ori,%20Alessandro&rft.genre=article&
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