Show simple item record

dc.contributor.author
Labouesse, Marie A.
dc.contributor.author
Polesel, Marcello
dc.contributor.author
Clementi, Elena
dc.contributor.author
Müller F, Flavia
dc.contributor.author
Markkanen, Enni
dc.contributor.author
Mouttet, Forouhar
dc.contributor.author
Cattaneo, Annamaria
dc.contributor.author
Richetto, Juliet
dc.date.accessioned
2018-06-14T12:35:02Z
dc.date.available
2018-06-09T07:29:23Z
dc.date.available
2018-06-14T12:35:02Z
dc.date.issued
2018-05-29
dc.identifier.issn
2045-2322
dc.identifier.other
10.1038/s41598-018-26631-x
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/268873
dc.identifier.doi
10.3929/ethz-b-000268873
dc.description.abstract
The medial prefrontal cortex (mPFC), master regulator of higher-order cognitive functions, is the only brain region that matures until late adolescence. During this period, the mPFC is sensitive to stressful events or suboptimal nutrition. For instance, high-fat diet (HFD) feeding during adolescence markedly impairs prefrontal-dependent cognition. It also provokes multiple changes at the cellular and synaptic scales within the mPFC, suggesting that major transcriptional events are elicited by HFD during this maturational period. The nature of this transcriptional reprogramming remains unknown, but may include epigenetic processes, in particular microRNAs, known to directly regulate synaptic functions. We used high–throughput screening in the adolescent mouse mPFC and identified 38 microRNAs differentially regulated by HFD, in particular mir-30e-5p. We used a luciferase assay to confirm the functional effect of mir-30e-5p on a chosen target: Ephrin-A3. Using global pathway analyses of predicted microRNA targets, we identified biological pathways putatively affected by HFD. Axon guidance was the top-1 pathway, validated by identifying gene expression changes of axon guidance molecules following HFD. Our findings delineate major microRNA transcriptional reprogramming within the mPFC induced by adolescent HFD. These results will help understanding the contribution of microRNAs in the emergence of cognitive deficits following early-life environmental events.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
MicroRNA expression profiling in the prefrontal cortex: Putative mechanisms for the cognitive effects of adolescent high fat feeding
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
Scientific Reports
ethz.journal.volume
8
en_US
ethz.journal.issue
1
en_US
ethz.journal.abbreviated
Sci Rep
ethz.pages.start
8344
en_US
ethz.size
14 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Inflammation-mediated epigenetic priming of neurodevelopmental disease
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.grant.agreementno
146217
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Projektförderung in Biologie und Medizin (Abteilung III)
ethz.date.deposited
2018-06-09T07:29:28Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2018-06-14T12:35:13Z
ethz.rosetta.lastUpdated
2021-02-15T00:22:25Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=MicroRNA%20expression%20profiling%20in%20the%20prefrontal%20cortex:%20Putative%20mechanisms%20for%20the%20cognitive%20effects%20of%20adolescent%20high%20fat%20feeding&rft.jtitle=Scientific%20Reports&rft.date=2018-05-29&rft.volume=8&rft.issue=1&rft.spage=8344&rft.issn=2045-2322&rft.au=Labouesse,%20Marie%20A.&Polesel,%20Marcello&Clementi,%20Elena&M%C3%BCller%20F,%20Flavia&Markkanen,%20Enni&rft.genre=article&rft_id=info:doi/10.1038/s41598-018-26631-x&
 Search print copy at ETH Library

Files in this item

Thumbnail

Publication type

Show simple item record