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dc.contributor.author
Fendt, Sarah-Maria
dc.contributor.author
Oliveira, Ana Paula
dc.contributor.author
Christen, Stefan
dc.contributor.author
Picotti, Paola
dc.contributor.author
Dechant, Reinhard Christoph
dc.contributor.author
Sauer, Uwe
dc.date.accessioned
2018-06-07T16:09:48Z
dc.date.available
2017-06-09T08:15:21Z
dc.date.available
2018-06-07T16:09:48Z
dc.date.issued
2010-01
dc.identifier.other
10.1038/msb.2010.91
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/26918
dc.identifier.doi
10.3929/ethz-b-000026918
dc.description.abstract
Which transcription factors control the distribution of metabolic fluxes under a given condition? We address this question by systematically quantifying metabolic fluxes in 119 transcription factor deletion mutants of Saccharomyces cerevisiae under five growth conditions. While most knockouts did not affect fluxes, we identified 42 condition‐dependent interactions that were mediated by a total of 23 transcription factors that control almost exclusively the cellular decision between respiration and fermentation. This relatively sparse, condition‐specific network of active metabolic control contrasts with the much larger gene regulation network inferred from expression and DNA binding data. Based on protein and transcript analyses in key mutants, we identified three enzymes in the tricarboxylic acid cycle as the key targets of this transcriptional control. For the transcription factor Gcn4, we demonstrate that this control is mediated through the PKA and Snf1 signaling cascade. The discrepancy between flux response predictions, based on the known regulatory network architecture and our functional 13C‐data, demonstrates the importance of identifying and quantifying the extent to which regulatory effectors alter cellular functions.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publ. Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-sa/3.0/
dc.subject
Metabolic flux
en_US
dc.subject
Omics data
en_US
dc.subject
Regulatory network
en_US
dc.subject
Transcription factor
en_US
dc.subject
Transcriptional regulation
en_US
dc.title
Unraveling condition-dependent networks of transcription factors that control metabolic pathway activity in yeast
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported
dc.date.published
2010-11-30
ethz.journal.title
Molecular Systems Biology
ethz.journal.volume
6
en_US
ethz.journal.issue
1
en_US
ethz.pages.start
432
en_US
ethz.size
11 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
004931271
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03595 - Peter, Matthias / Peter, Matthias
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03663 - Aebersold, Rudolf / Aebersold, Rudolf
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03713 - Sauer, Uwe / Sauer, Uwe
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03595 - Peter, Matthias / Peter, Matthias
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03663 - Aebersold, Rudolf / Aebersold, Rudolf
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology::03713 - Sauer, Uwe / Sauer, Uwe
ethz.date.deposited
2017-06-09T08:15:35Z
ethz.source
ECIT
ethz.identifier.importid
imp59364d70188a215316
ethz.ecitpid
pub:45656
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-20T14:46:25Z
ethz.rosetta.lastUpdated
2018-11-07T11:31:24Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Unraveling%20condition-dependent%20networks%20of%20transcription%20factors%20that%20control%20metabolic%20pathway%20activity%20in%20yeast&rft.jtitle=Molecular%20Systems%20Biology&rft.date=2010-01&rft.volume=6&rft.issue=1&rft.spage=432&rft.au=Fendt,%20Sarah-Maria&Oliveira,%20Ana%20Paula&Christen,%20Stefan&Picotti,%20Paola&Dechant,%20Reinhard%20Christoph&rft.genre=article&
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