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dc.contributor.author
Chawla, Ketki
dc.contributor.author
Burgel, Sebastian C.
dc.contributor.author
Schmidt, Gregor W.
dc.contributor.author
Kaltenbach, Hans-Michael
dc.contributor.author
Rudolf, Fabian
dc.contributor.author
Frey, Olivier
dc.contributor.author
Hierlemann, Andreas
dc.date.accessioned
2018-06-25T16:30:47Z
dc.date.available
2018-06-20T17:13:57Z
dc.date.available
2018-06-25T16:30:47Z
dc.date.issued
2018-05-24
dc.identifier.issn
2096-1030
dc.identifier.issn
2055-7434
dc.identifier.other
10.1038/s41378-018-0006-5
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/271144
dc.identifier.doi
10.3929/ethz-b-000271144
dc.description.abstract
Growth rate is a widely studied parameter for various cell-based biological studies. Growth rates of cell populations can be monitored in chemostats and micro-chemostats, where nutrients are continuously replenished. Here, we present an integrated microfluidic platform that enables long-term culturing of non-adherent cells as well as parallel and mutually independent continuous monitoring of (i) growth rates of cells by means of impedance measurements and of (ii) specific other cellular events by means of high-resolution optical or fluorescence microscopy. Yeast colonies were grown in a monolayer under culturing pads, which enabled high-resolution microscopy, as all cells were in the same focal plane. Upon cell growth and division, cells leaving the culturing area passed over a pair of electrodes and were counted through impedance measurements. The impedance data could then be used to directly determine the growth rates of the cells in the culturing area. The integration of multiple culturing chambers with sensing electrodes enabled multiplexed long-term monitoring of growth rates of different yeast strains in parallel. As a demonstration, we modulated the growth rates of engineered yeast strains using calcium. The results indicated that impedance measurements provide a label-free readout method to continuously monitor the changes in the growth rates of the cells without compromising high-resolution optical imaging of single cells.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Springer
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Integrating impedance-based growth-rate monitoring into a microfluidic cell culture platform for live-cell microscopy
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2018-05-24
ethz.journal.title
Microsystems & Nanoengineering
ethz.journal.volume
4
en_US
ethz.pages.start
8
en_US
ethz.size
12 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03699 - Stelling, Jörg / Stelling, Jörg
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03684 - Hierlemann, Andreas / Hierlemann, Andreas
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03699 - Stelling, Jörg / Stelling, Jörg
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03684 - Hierlemann, Andreas / Hierlemann, Andreas
ethz.date.deposited
2018-06-20T17:14:12Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2018-06-25T16:30:59Z
ethz.rosetta.lastUpdated
2019-02-03T03:30:47Z
ethz.rosetta.versionExported
true
ethz.COinS
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