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dc.contributor.author
Zampa, Federico
dc.contributor.author
Bicker, Silvia
dc.contributor.author
Schratt, Gerhard
dc.date.accessioned
2018-06-25T15:39:28Z
dc.date.available
2018-06-22T04:37:50Z
dc.date.available
2018-06-25T15:39:28Z
dc.date.issued
2018-06-11
dc.identifier.other
10.3389/fnmol.2018.00171
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/271468
dc.identifier.doi
10.3929/ethz-b-000271468
dc.description.abstract
microRNAs (miRNAs) have emerged as critical regulators of neuronal dendrite development. Specific precursor (pre-)miRNAs are actively transported to dendrites, but whether this process is regulated by neuronal activity and involved in activitydependent dendritogenesis is unknown. Here we show that BDNF, a neurotrophin that is released in response to increased neuronal activity, promotes dendritic accumulation of pre-miR-134. Dendritic accumulation, but not transcription of pre-miR-134, is abrogated by treatment of neurons with the NMDA receptor (NMDAR) antagonist APV. Furthermore, APV interferes with BDNF-mediated repression of the known miR-134 target Pumilio 2 (Pum2) in a miR-134 binding site-specific manner. At the functional level, both APV treatment and knockdown of the pre-miR-134 transport protein DHX36 antagonize BDNF-induced dendritogenesis. These effects are likely mediated by reduced dendritic miR-134 activity, since both transfection of a synthetic miR-134 duplex or of a dendritically targeted pre-miR-134-181a chimera rescues BDNF-dependent dendritogenesis in the presence of APV. In conclusion, we have identified a novel NMDAR-dependent mechanism involved in the activity-dependent control of miRNA function during neuronal development.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Frontiers
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
dendritogenesis
en_US
dc.subject
microRNA
en_US
dc.subject
neuronal activity
en_US
dc.subject
BDNF
en_US
dc.subject
NMDA receptor
en_US
dc.subject
RNA transport
en_US
dc.subject
neuronal development
en_US
dc.title
Activity-Dependent Pre-miR-134 Dendritic Localization Is Required for Hippocampal Neuron Dendritogenesis
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
Frontiers in Molecular Neuroscience
ethz.journal.volume
11
en_US
ethz.pages.start
171
en_US
ethz.size
14 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Lausanne
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02542 - Institut für Neurowissenschaften / Institute for Neuroscience::09498 - Schratt, Gerhard / Schratt, Gerhard
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02070 - Dep. Gesundheitswiss. und Technologie / Dep. of Health Sciences and Technology::02542 - Institut für Neurowissenschaften / Institute for Neuroscience::09498 - Schratt, Gerhard / Schratt, Gerhard
ethz.date.deposited
2018-06-22T04:38:35Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2018-06-25T15:39:39Z
ethz.rosetta.lastUpdated
2019-01-02T13:17:01Z
ethz.rosetta.versionExported
true
ethz.COinS
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