Show simple item record

dc.contributor.author
Kroschwald, Sonja
dc.contributor.author
Munder, Matthias C.
dc.contributor.author
Maharana, Shovamayee
dc.contributor.author
Franzmann, Titus M.
dc.contributor.author
Richter, Doris
dc.contributor.author
Ruer, Martine
dc.contributor.author
Hyman, Anthony A.
dc.contributor.author
Alberti, Simon
dc.date.accessioned
2018-06-26T14:39:00Z
dc.date.available
2018-06-22T13:52:06Z
dc.date.available
2018-06-26T14:39:00Z
dc.date.issued
2018-06-12
dc.identifier.issn
2666-3864
dc.identifier.issn
2211-1247
dc.identifier.other
10.1016/j.celrep.2018.05.041
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/271705
dc.identifier.doi
10.3929/ethz-b-000271705
dc.description.abstract
How cells adapt to varying environmental conditions is largely unknown. Here, we show that, in budding yeast, the RNA-binding and stress granule protein Pub1 has an intrinsic property to form condensates upon starvation or heat stress and that condensate formation is associated with cell-cycle arrest. Release from arrest coincides with condensate dissolution, which takes minutes (starvation) or hours (heat shock). In vitro reconstitution reveals that the different dissolution rates of starvation- and heat-induced condensates are due to their different material properties: starvation-induced Pub1 condensates form by liquid-liquid demixing and subsequently convert into reversible gel-like particles; heat-induced condensates are more solid-like and require chaperones for disaggregation. Our data suggest that different physiological stresses, as well as stress durations and intensities, induce condensates with distinct physical properties and thereby define different modes of stress adaptation and rates of recovery.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Elsevier
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject
phase separation
en_US
dc.subject
condensate
en_US
dc.subject
phase transition
en_US
dc.subject
stress granule
en_US
dc.subject
stress response
en_US
dc.subject
molecular chaperone
en_US
dc.subject
Hsp104
en_US
dc.subject
protein aggregation
en_US
dc.subject
cytosolic pH
en_US
dc.title
Different Material States of Pub1 Condensates Define Distinct Modes of Stress Adaptation and Recovery
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
dc.date.published
2018-06-12
ethz.journal.title
Cell Reports
ethz.journal.volume
23
en_US
ethz.journal.issue
11
en_US
ethz.journal.abbreviated
Cell Rep
ethz.pages.start
3327
en_US
ethz.pages.end
3339
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Amsterdam
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2018-06-22T13:52:11Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2018-06-26T14:39:12Z
ethz.rosetta.lastUpdated
2022-03-28T20:32:00Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Different%20Material%20States%20of%20Pub1%20Condensates%20Define%20Distinct%20Modes%20of%20Stress%20Adaptation%20and%20Recovery&rft.jtitle=Cell%20Reports&rft.date=2018-06-12&rft.volume=23&rft.issue=11&rft.spage=3327&rft.epage=3339&rft.issn=2666-3864&2211-1247&rft.au=Kroschwald,%20Sonja&Munder,%20Matthias%20C.&Maharana,%20Shovamayee&Franzmann,%20Titus%20M.&Richter,%20Doris&rft.genre=article&rft_id=info:doi/10.1016/j.celrep.2018.05.041&
 Search print copy at ETH Library

Files in this item

Thumbnail

Publication type

Show simple item record