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dc.contributor.author
Dultz, Elisa
dc.contributor.author
Mancini, Roberta
dc.contributor.author
Polles, Guido
dc.contributor.author
Vallotton, Pascal
dc.contributor.author
Alber, Frank
dc.contributor.author
Weis, Karsten
dc.date.accessioned
2018-09-13T15:00:00Z
dc.date.available
2018-08-01T07:04:05Z
dc.date.available
2018-08-03T09:20:59Z
dc.date.available
2018-09-13T15:00:00Z
dc.date.issued
2018-07-15
dc.identifier.other
10.1091/mbc.E17-11-0648
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/279739
dc.identifier.doi
10.3929/ethz-b-000279739
dc.description.abstract
Chromatin organization is highly dynamic and regulates transcription. Upon transcriptional activation, chromatin is remodeled and referred to as “open,” but quantitative and dynamic data of this decompaction process are lacking. Here, we have developed a quantitative high resolution–microscopy assay in living yeast cells to visualize and quantify chromatin dynamics using the GAL7-10-1 locus as a model system. Upon transcriptional activation of these three clustered genes, we detect an increase of the mean distance across this locus by >100 nm. This decompaction is linked to active transcription but is not sensitive to the histone deacetylase inhibitor trichostatin A or to deletion of the histone acetyl transferase Gcn5. In contrast, the deletion of SNF2 (encoding the ATPase of the SWI/SNF chromatin remodeling complex) or the deactivation of the histone chaperone complex FACT lead to a strongly reduced decompaction without significant effects on transcriptional induction in FACT mutants. Our findings are consistent with nucleosome remodeling and eviction activities being major contributors to chromatin reorganization during transcription but also suggest that transcription can occur in the absence of detectable decompaction.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
American Society for Cell Biology
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-sa/3.0/
dc.title
Quantitative imaging of chromatin decompaction in living cells
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported
dc.date.published
2018-05-12
ethz.journal.title
Molecular Biology of the Cell
ethz.journal.volume
29
en_US
ethz.journal.issue
14
en_US
ethz.pages.start
1763
en_US
ethz.pages.end
1777
en_US
ethz.size
15 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Structure and Function of the Nuclear Pore Complex
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Bethesda, MD
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::09464 - Weis, Karsten / Weis, Karsten
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::09464 - Weis, Karsten / Weis, Karsten
ethz.grant.agreementno
159731
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Projektförderung in Biologie und Medizin (Abteilung III)
ethz.date.deposited
2018-08-01T07:04:18Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.date.embargoend
2018-09-15
ethz.rosetta.installDate
2018-08-03T09:21:03Z
ethz.rosetta.lastUpdated
2018-12-02T13:35:51Z
ethz.rosetta.versionExported
true
ethz.COinS
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