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dc.contributor.author
Ryu, Hyunryul
dc.contributor.author
Chung, Minhwan
dc.contributor.author
Song, Jiyoung
dc.contributor.author
Lee, Sung S.
dc.contributor.author
Pertz, Olivier
dc.contributor.author
Jeon, Noo L.
dc.date.accessioned
2018-08-07T13:34:15Z
dc.date.available
2018-08-02T05:07:31Z
dc.date.available
2018-08-07T13:34:15Z
dc.date.issued
2018
dc.identifier.other
10.1038/s41598-018-28873-1
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/279986
dc.identifier.doi
10.3929/ethz-b-000279986
dc.description.abstract
Extracellular response kinase (ERK) is one of the key regulator of cell fate, such as proliferation, differentiation and cell migration. Here, we propose a novel experimental pipeline to learn ERK kinetics by temporal growth factor (GF) stimulation. High signal-to-noise ratio of genetically encoded Fluorescence resonance energy transfer (FRET) biosensor enables to get a large number of single-cell ERK activity at each time point, while computer-controlled microfluidics fine-tune the temporal stimulation. Using this platform, we observed that static Epidermal growth factor (EGF) stimulation led to transient ERK activation with a significant cell-to-cell variation, while dynamic stimulation of 3′ EGF pulse led to faster adaptation kinetics with no discrepancy. Multiple EGF pulses retriggered ERK activity with respect to frequency of stimulation. We also observed oscillation of ERK activity of each cell at basal state. Introducing of Mitogen-activated protein kinase kinase (MEK) inhibitor, U0126, was not only dropping the average of basal activity for 7.5%, but also diminishing oscillatory behavior. Activity level raised up when inhibitor was removed, followed by transient peak of ERK kinetics. We expect this platform to probe Mitogen-associated protein kinase (MAPK) signaling network for systems biology research at single cellular level.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Integrated Platform for Monitoring Single-cell MAPK Kinetics in Computer-controlled Temporal Stimulations
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2018-07-24
ethz.journal.title
Scientific Reports
ethz.journal.volume
8
en_US
ethz.pages.start
11126
en_US
ethz.size
7 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung & Wirtschaftsbez. / Domain VP Research & Corporate Relations::02891 - ScopeM / ScopeM
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00003 - Schulleitung und Dienste::00022 - Bereich VP Forschung & Wirtschaftsbez. / Domain VP Research & Corporate Relations::02891 - ScopeM / ScopeM
ethz.date.deposited
2018-08-02T05:07:44Z
ethz.source
WOS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2018-08-07T13:34:30Z
ethz.rosetta.lastUpdated
2019-02-03T05:06:22Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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