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dc.contributor.author
Teräväinen, Terhi P.
dc.contributor.author
Myllymäki, Satu M.
dc.contributor.author
Friedrichs, Jens
dc.contributor.author
Strohmeyer, Nico
dc.contributor.author
Moyano, Jose V.
dc.contributor.author
Wu, Chuanyue
dc.contributor.author
Matlin, Karl S.
dc.contributor.author
Muller, Daniel J.
dc.contributor.author
Manninen, Aki
dc.date.accessioned
2018-09-06T07:17:30Z
dc.date.available
2018-08-13T05:48:03Z
dc.date.available
2018-08-13T05:54:42Z
dc.date.available
2018-09-06T07:15:38Z
dc.date.available
2018-09-06T07:17:30Z
dc.date.issued
2013-08-19
dc.identifier.issn
1932-6203
dc.identifier.other
10.1371/journal.pone.0071485
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/282032
dc.identifier.doi
10.3929/ethz-b-000282032
dc.description.abstract
The properties of epithelial cells within tissues are regulated by their immediate microenvironment, which consists of neighboring cells and the extracellular matrix (ECM). Integrin heterodimers orchestrate dynamic assembly and disassembly of cell-ECM connections and thereby convey biochemical and mechanical information from the ECM into cells. However, the specific contributions and functional hierarchy between different integrin heterodimers in the regulation of focal adhesion dynamics in epithelial cells are incompletely understood. Here, we have studied the functions of RGD-binding αV-integrins in a Madin Darby Canine Kidney (MDCK) cell model and found that αV-integrins regulate the maturation of focal adhesions (FAs) and cell spreading. αV-integrin-deficient MDCK cells bound collagen I (Col I) substrate via α2β1-integrins but failed to efficiently recruit FA components such as talin, focal adhesion kinase (FAK), vinculin and integrin-linked kinase (ILK). The apparent inability to mature α2β1-integrin-mediated FAs and link them to cellular actin cytoskeleton led to disrupted mechanotransduction in αV-integrin deficient cells seeded onto Col I substrate.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.title
αV-integrins are required for mechanotransduction in MDCK epithelial cells
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
ethz.journal.title
PLoS ONE
ethz.journal.volume
8
en_US
ethz.journal.issue
8
en_US
ethz.journal.abbreviated
PLoS ONE
ethz.pages.start
e71485
en_US
ethz.size
15 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.nebis
006206116
ethz.publication.place
San Francisco, CA, USA
en_US
ethz.publication.status
published
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03870 - Müller, Daniel J. / Müller, Daniel J.
ethz.identifier.orcidWorkCode
12672726
ethz.date.deposited
2017-06-10T23:12:58Z
ethz.source
ECIT
ethz.source
FORM
ethz.identifier.importid
imp59365121e449561455
ethz.ecitpid
pub:116697
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2018-08-13T05:48:06Z
ethz.rosetta.lastUpdated
2018-11-08T01:39:27Z
ethz.rosetta.versionExported
true
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/73741
dc.identifier.olduri
http://hdl.handle.net/20.500.11850/281645
ethz.COinS
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