A molecular mechanism for the enzymatic methylation of nitrogen atoms within peptide bonds
Velden, Niels S. van der
Shiran, Sally L.
Imani, Aman S.
Freeman, Michael F.
Naismith, James H.
- Journal Article
Rights / licenseCreative Commons Attribution 4.0 International
The peptide bond, the defining feature of proteins, governs peptide chemistry by abolishing nucleophilicity of the nitrogen. This and the planarity of the peptide bond arise from the delocalization of the lone pair of electrons on the nitrogen atom into the adjacent carbonyl. While chemical methylation of an amide bond uses a strong base to generate the imidate, OphA, the precursor protein of the fungal peptide macrocycle omphalotin A, self-hypermethylates amides at pH 7 using S-adenosyl methionine (SAM) as cofactor. The structure of OphA reveals a complex catenane-like arrangement in which the peptide substrate is clamped with its amide nitrogen aligned for nucleophilic attack on the methyl group of SAM. Biochemical data and computational modeling suggest a base-catalyzed reaction with the protein stabilizing the reaction intermediate. Backbone N-methylation of peptides enhances their protease resistance and membrane permeability, a property that holds promise for applications to medicinal chemistry. Show more
Journal / seriesScience Advances
Pages / Article No.
Organisational unit02520 - Institut für Mikrobiologie / Institute of Microbiology
02030 - Dep. Biologie / Dep. of Biology
09458 - Riniker, Sereina Z. / Riniker, Sereina Z.
03408 - Aebi, Markus / Aebi, Markus
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