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dc.contributor.author
Panagiotakopoulou, Magdalini
dc.contributor.author
Lendenmann, Tobias
dc.contributor.author
Pramotton, Francesca M.
dc.contributor.author
Giampietro, Costanza
dc.contributor.author
Stefopoulos, Georgios
dc.contributor.author
Poulikakos, Dimos
dc.contributor.author
Ferrari, Aldo
dc.date.accessioned
2018-12-19T13:47:45Z
dc.date.available
2018-09-11T06:07:38Z
dc.date.available
2018-09-12T12:26:24Z
dc.date.available
2018-09-12T12:28:28Z
dc.date.available
2018-09-28T12:57:59Z
dc.date.available
2018-11-07T09:02:40Z
dc.date.available
2018-11-07T09:36:20Z
dc.date.available
2018-12-19T13:47:45Z
dc.date.issued
2018-10-15
dc.identifier.other
10.1091/mbc.E17-12-0726
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/288281
dc.identifier.doi
10.3929/ethz-b-000288281
dc.description.abstract
The generation of traction forces and their transmission to the extracellular environment supports the disseminative migration of cells from a primary tumor. In cancer cells, the periodic variation of nuclear stiffness during the cell cycle provides a functional link between efficient translocation and proliferation. However, the mechanical framework completing this picture remains unexplored. Here, the Fucci2 reporter was expressed in various human epithelial cancer cells to resolve their cell cycle phase transition. The corresponding tractions were captured by a recently developed reference-free confocal traction-force microscopy platform. The combined approach was conducive to the analysis of phase-dependent force variation at the level of individual integrin contacts. Detected forces were invariably higher in the G1 and early S phases than in the ensuing late S/G2, and locally colocalized with high levels of paxillin phosphorylation. Perturbation of paxillin phosphorylation at focal adhesions, obtained through the biochemical inhibition of focal adhesion kinase (FAK) or the transfection of nonphosphorylatable or phosphomimetic paxillin mutants, significantly diminished the force transmitted to the substrate. These data demonstrate a reproducible modulation of force transmission during the cell cycle progression of cancer cells, instrumental to their invasion of dense environments. In addition, they delineate a model in which paxillin phosphorylation supports the mechanical maturation of adhesions relaying forces to the substrate.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
American Society for Cell Biology
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-sa/3.0/
dc.title
Cell cycle-dependent force transmission in cancer cells
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported
dc.date.published
2018-08-16
ethz.journal.title
Molecular Biology of the Cell
ethz.journal.volume
29
en_US
ethz.journal.issue
21
en_US
ethz.pages.start
2528
en_US
ethz.pages.end
2539
en_US
ethz.size
12 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.grant
Novel Microengineered Platform for the Study of Interstitial Cell Migration
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Bethesda, MD
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02130 - Dep. Maschinenbau und Verfahrenstechnik / Dep. of Mechanical and Process Eng.::02668 - Inst. f. Energie- und Verfahrenstechnik / Inst. Energy and Process Engineering::03462 - Poulikakos, Dimos / Poulikakos, Dimos
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02130 - Dep. Maschinenbau und Verfahrenstechnik / Dep. of Mechanical and Process Eng.::02668 - Inst. f. Energie- und Verfahrenstechnik / Inst. Energy and Process Engineering::03462 - Poulikakos, Dimos / Poulikakos, Dimos
en_US
ethz.grant.agreementno
146898
ethz.grant.fundername
SNF
ethz.grant.funderDoi
10.13039/501100001711
ethz.grant.program
Projektförderung in Mathematik, Natur- und Ingenieurwissenschaften (Abteilung II)
ethz.date.deposited
2018-09-11T06:07:39Z
ethz.source
FORM
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.date.embargoend
2018-12-15
ethz.rosetta.installDate
2018-11-07T09:36:28Z
ethz.rosetta.lastUpdated
2021-02-15T03:08:39Z
ethz.rosetta.versionExported
true
ethz.COinS
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