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dc.contributor.author
Bull, Simon E.
dc.contributor.author
Seung, David
dc.contributor.author
Chanez, Christelle
dc.contributor.author
Mehta, Devang
dc.contributor.author
Kuon, Joel‑Elias
dc.contributor.author
Truernit, Elisabeth
dc.contributor.author
Hochmuth, Anton
dc.contributor.author
Zurkirchen, Irene
dc.contributor.author
Zeeman, Samuel C.
dc.contributor.author
Gruissem, Wilhelm
dc.contributor.author
Vanderschuren, Hervé
dc.date.accessioned
2018-09-18T10:54:23Z
dc.date.available
2018-09-15T07:08:25Z
dc.date.available
2018-09-18T10:54:23Z
dc.date.issued
2018-09-05
dc.identifier.issn
2375-2548
dc.identifier.other
10.1126/sciadv.aat6086
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/289809
dc.identifier.doi
10.3929/ethz-b-000289809
dc.description.abstract
Crop diversification required to meet demands for food security and industrial use is often challenged by breeding time and amenability of varieties to genome modification. Cassava is one such crop. Grown for its large starch-rich storage roots, it serves as a staple food and a commodity in the multibillion-dollar starch industry. Starch is composed of the glucose polymers amylopectin and amylose, with the latter strongly influencing the physicochemical properties of starch during cooking and processing. We demonstrate that CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9)–mediated targeted mutagenesis of two genes involved in amylose biosynthesis, PROTEIN TARGETING TO STARCH (PTST1) or GRANULE BOUND STARCH SYNTHASE (GBSS), can reduce or eliminate amylose content in root starch. Integration of the Arabidopsis FLOWERING LOCUS T gene in the genome-editing cassette allowed us to accelerate flowering—an event seldom seen under glasshouse conditions. Germinated seeds yielded S1, a transgene-free progeny that inherited edited genes. This attractive new plant breeding technique for modified cassava could be extended to other crops to provide a suite of novel varieties with useful traits for food and industrial applications.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
AAAS
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc/4.0/
dc.title
Accelerated ex situ breeding of GBSS- and PTST1-edited cassava for modified starch
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial 4.0 International
ethz.journal.title
Science Advances
ethz.journal.volume
4
en_US
ethz.journal.issue
9
en_US
ethz.journal.abbreviated
Sci Adv
ethz.pages.start
eaat6086
en_US
ethz.size
12 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.publication.place
Washington, DC
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03969 - Studer, Bruno / Studer, Bruno
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02541 - Institut für Molekulare Pflanzenbiologie / Institute of Molecular Plant Biology::03707 - Zeeman, Samuel C. / Zeeman, Samuel C.
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02703 - Institut für Agrarwissenschaften / Institute of Agricultural Sciences::03969 - Studer, Bruno / Studer, Bruno
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02541 - Institut für Molekulare Pflanzenbiologie / Institute of Molecular Plant Biology::03707 - Zeeman, Samuel C. / Zeeman, Samuel C.
ethz.date.deposited
2018-09-15T07:08:39Z
ethz.source
SCOPUS
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2018-09-18T10:54:28Z
ethz.rosetta.lastUpdated
2021-02-15T01:47:17Z
ethz.rosetta.versionExported
true
ethz.COinS
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