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dc.contributor.author
Nylund, Reetta
dc.contributor.author
Kuster, Niels
dc.contributor.author
Leszczynski, Dariusz
dc.date.accessioned
2019-10-08T15:50:18Z
dc.date.available
2017-06-09T09:08:31Z
dc.date.available
2019-10-08T15:50:18Z
dc.date.issued
2010
dc.identifier.issn
1477-5956
dc.identifier.other
10.1186/1477-5956-8-52
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/29483
dc.identifier.doi
10.3929/ethz-b-000029483
dc.description.abstract
Background Use of mobile phones has widely increased over the past decade. However, in spite of the extensive research, the question of potential health effects of the mobile phone radiation remains unanswered. We have earlier proposed, and applied, proteomics as a tool to study biological effects of the mobile phone radiation, using as a model human endothelial cell line EA.hy926. Exposure of EA.hy926 cells to 900 MHz GSM radiation has caused statistically significant changes in expression of numerous proteins. However, exposure of EA.hy926 cells to 1800 MHz GSM signal had only very small effect on cell proteome, as compared with 900 MHz GSM exposure. In the present study, using as model human primary endothelial cells, we have examined whether exposure to 1800 MHz GSM mobile phone radiation can affect cell proteome. Results Primary human umbilical vein endothelial cells and primary human brain microvascular endothelial cells were exposed for 1 hour to 1800 MHz GSM mobile phone radiation at an average specific absorption rate of 2.0 W/kg. The cells were harvested immediately after the exposure and the protein expression patterns of the sham-exposed and radiation-exposed cells were examined using two dimensional difference gel electrophoresis-based proteomics (2DE-DIGE). There were observed numerous differences between the proteomes of human umbilical vein endothelial cells and human brain microvascular endothelial cells (both sham-exposed). These differences are most likely representing physiological differences between endothelia in different vascular beds. However, the exposure of both types of primary endothelial cells to mobile phone radiation did not cause any statistically significant changes in protein expression. Conclusions Exposure of primary human endothelial cells to the mobile phone radiation, 1800 MHz GSM signal for 1 hour at an average specific absorption rate of 2.0 W/kg, does not affect protein expression, when the proteomes were examined immediately after the end of the exposure and when the false discovery rate correction was applied to analysis. This observation agrees with our earlier study showing that the 1800 MHz GSM radiation exposure had only very limited effect on the proteome of human endothelial cell line EA.hy926, as compared with the effect of 900 MHz GSM radiation.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
BioMed Central
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/2.0/
dc.title
Analysis of proteome response to the mobile phone radiation in two types of human primary endothelial cells
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 2.0 Generic
dc.date.published
2010-10-18
ethz.journal.title
Proteome Science
ethz.journal.volume
8
en_US
ethz.journal.abbreviated
Proteome sci.
ethz.pages.start
52
en_US
ethz.size
7 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.nebis
004405180
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
03228 - Fichtner, Wolfgang
en_US
ethz.leitzahl.certified
03228 - Fichtner, Wolfgang
ethz.date.deposited
2017-06-09T09:08:44Z
ethz.source
ECIT
ethz.identifier.importid
imp59364d9a8e91a58457
ethz.ecitpid
pub:48932
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-19T10:51:30Z
ethz.rosetta.lastUpdated
2019-10-08T15:50:31Z
ethz.rosetta.versionExported
true
ethz.COinS
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