Attempts to implement CRISPR/Cas9 for genome editing in the oomycete Phytophthora infestans
- Working Paper
Few techniques have revolutionized the molecular biology field as much as genome editing using CRISPR/Cas9. Recently, a CRISPR/Cas9 system has been developed for Phytophthora sojae, and since then it has been employed in two other Phytophthora spp. Here, we report our progress on efforts to establish the system in Phytophthora infestans. Using the original constructs as developed for P. sojae, we did not obtain any transformants displaying a mutagenized target gene. We made several modifications to the CRISPR/Cas9 system to pinpoint the reason for failure and also explored the delivery of pre-assembled ribonucleoprotein complexes. This report summarizes an extensive experimental effort pursuing the application of a CRISPR/Cas9 system for targeted mutagenesis in P. infestans and concludes with suggestions for future directions. Show more
Journal / seriesbioRxiv
Pages / Article No.
PublisherCold Spring Harbor Laboratory
Organisational unit09625 - Crowther, Thomas Ward / Crowther, Thomas Ward
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